Mouse Calreticulin (CRT) ELISA Kit | CRT elisa kit

Mouse Calreticulin (CRT) ELISA Kit

Cat. Num. AAA2023166

• Gene Names: CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
• Reactivity: Mouse
• Synonyms: Calreticulin (CRT); Mouse Calreticulin (CRT) ELISA Kit; CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60; CRT elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT).; No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.
• Sequence Length: 417

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CRT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CRT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CRT elisa kit

Signal transduction; Metabolic pathway; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4757900
• NCBI GeneID: 811
• NCBI Accession #: NP_004334.1
• NCBI GenBank Nucleotide #: NM_004343.3
• UniProt Accession #: P27797; Q6IAT4; Q9UDG2
• Molecular Weight: 48,142 Da
• NCBI Official Full Name: calreticulin
• NCBI Official Synonym Full Names: calreticulin
• NCBI Official Symbol: CALR
• NCBI Official Synonym Symbols: RO; CRT; SSA; cC1qR; HEL-S-99n
• NCBI Protein Information: calreticulin
• UniProt Protein Name: Calreticulin
• Protein Family: Chloroquine resistance transporter
• UniProt Gene Name: CALR
• UniProt Synonym Gene Names: CRTC; ERp60
• UniProt Entry Name: CALR_HUMAN

NCBI Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Uniprot Description

Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family.

Protein type: Secreted, signal peptide; Calcium-binding; Motility/polarity/chemotaxis; Secreted; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: Golgi apparatus; extracellular space; focal adhesion; sarcoplasmic reticulum lumen; smooth endoplasmic reticulum; cell surface; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region; acrosome; cytosol; polysome; proteinaceous extracellular matrix; membrane; perinuclear region of cytoplasm; cytoplasm; intracellular; nucleus; external side of plasma membrane

Molecular Function: hormone binding; zinc ion binding; complement component C1q binding; unfolded protein binding; calcium ion binding; peptide binding; integrin binding; mRNA binding; protein binding; DNA binding; androgen receptor binding; ubiquitin protein ligase binding; iron ion binding; chaperone binding; glycoprotein binding; carbohydrate binding

Biological Process: protein maturation via protein folding; protein folding; regulation of meiosis; negative regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus; cardiac muscle cell differentiation; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of transcription, DNA-dependent; positive regulation of phagocytosis; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; cortical actin cytoskeleton organization and biogenesis; protein export from nucleus; cell cycle arrest; response to drug; receptor-mediated endocytosis; negative regulation of steroid hormone receptor signaling pathway; protein stabilization; unfolded protein response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell cycle; response to testosterone stimulus; post-translational protein modification; peptide antigen assembly with MHC class I protein complex; cellular calcium ion homeostasis; negative regulation of retinoic acid receptor signaling pathway; negative regulation of neuron differentiation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sequestering of calcium ion; glucocorticoid receptor signaling pathway; negative regulation of translation; spermatogenesis; protein amino acid N-linked glycosylation via asparagine; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication

Disease: Myelofibrosis; Thrombocythemia 1