Typical Testing Data/Standard Curve (for reference only)
Mouse Butyrophilin subfamily 2 member A2 (BTN2A2) ELISA Kit | BTN2A2 elisa kit
Mouse Butyrophilin subfamily 2 member A2 (BTN2A2) ELISA Kit
Gene Names
BTN2A1; BTN2A2
Reactivity
Mouse
Synonyms
Butyrophilin subfamily 2 member A2 (BTN2A2); Mouse Butyrophilin subfamily 2 member A2 (BTN2A2) ELISA Kit; BTN2A2 elisa kit
Reactivity
Mouse
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BTN2A2 elisa kit
Intended Uses: This BTN2A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BTN2A2. This ELISA kit for research use only!
Principle of the Assay: BTN2A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BTN2A2 antibody and an BTN2A2-HRP conjugate. The assay sample and buffer are incubated together with BTN2A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTN2A2 concentration since BTN2A2 from samples and BTN2A2-HRP conjugate compete for the anti-BTN2A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BTN2A2 from the sample, fewer sites are left to bind BTN2A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTN2A2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BTN2A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BTN2A2 antibody and an BTN2A2-HRP conjugate. The assay sample and buffer are incubated together with BTN2A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTN2A2 concentration since BTN2A2 from samples and BTN2A2-HRP conjugate compete for the anti-BTN2A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BTN2A2 from the sample, fewer sites are left to bind BTN2A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTN2A2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BTN2A2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,060 Da
NCBI Official Full Name
butyrophilin subfamily 2 member A2
NCBI Official Symbol
BTN2A1
NCBI Official Synonym Symbols
BTN2A2
NCBI Protein Information
butyrophilin subfamily 2 member A2
UniProt Protein Name
Butyrophilin subfamily 2 member A2
Protein Family
UniProt Gene Name
BTN2A2
UniProt Entry Name
BT2A2_PONAB
Uniprot Description
Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
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Product Notes
The Mouse BTN2A2 btn2a2 (Catalog #AAA7234859) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234859 ELISA Kit recognizes Mouse BTN2A2. It is sometimes possible for the material contained within the vial of "Butyrophilin subfamily 2 member A2 (BTN2A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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