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Typical Testing Data/Standard Curve (for reference only)

Mouse Beclin1, BECN1 ELISA Kit | BECN1 elisa kit

Mouse Beclin1, BECN1 ELISA Kit

Gene Names
becn1.L; becn1; beclin1
Reactivity
Mouse
Synonyms
Beclin1; BECN1; Mouse Beclin1; BECN1 ELISA Kit; BECN1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
445
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 15ng/ml
Sensitivity
0.017ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BECN1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Beclin1 (also known as BECN1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse BECN1 antibody. BECN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse BECN1 Antibody is added and binds to BECN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BECN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse BECN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,294 Da
NCBI Official Full Name
beclin-1
NCBI Official Synonym Full Names
beclin 1, autophagy related L homeolog
NCBI Official Symbol
becn1.L
NCBI Official Synonym Symbols
becn1; beclin1
NCBI Protein Information
beclin-1
UniProt Protein Name
Beclin-1
Protein Family
UniProt Gene Name
becn1

Uniprot Description

Plays a central role in autophagy (). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (). Involved in endocytosis including endosome formation in neuronal cells ().

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Product Notes

The Mouse BECN1 becn1 (Catalog #AAA165714) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA165714 ELISA Kit recognizes Mouse BECN1. It is sometimes possible for the material contained within the vial of "Beclin1, BECN1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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