Mouse AP-4 complex subunit mu-1 (AP4M1) ELISA Kit | AP4M1 elisa kit

Mouse AP-4 complex subunit mu-1 (AP4M1) ELISA Kit

Cat. Num. AAA7246363

• Gene Names: AP4M1; MU-4; CPSQ3; SPG50; MU-ARP2
• Reactivity: Mouse
• Synonyms: AP-4 complex subunit mu-1 (AP4M1); Mouse AP-4 complex subunit mu-1 (AP4M1) ELISA Kit; AP4M1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of AP4M1. No significant cross-reactivity or interference between AP4M1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AP4M1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for AP4M1 elisa kit

Intended Uses: This AP4M1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AP4M1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: AP4M1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AP4M1 antibody and an AP4M1-HRP conjugate. The assay sample and buffer are incubated together with AP4M1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AP4M1 concentration since AP4M1 from samples and AP4M1-HRP conjugate compete for the anti-AP4M1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AP4M1 from the sample, fewer sites are left to bind AP4M1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AP4M1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14917111
• NCBI GeneID: 9179
• NCBI Accession #: NP_004713.2
• NCBI GenBank Nucleotide #: NM_004722.3
• Molecular Weight: 49,977 Da
• NCBI Official Full Name: AP-4 complex subunit mu-1
• NCBI Official Synonym Full Names: adaptor-related protein complex 4, mu 1 subunit
• NCBI Official Symbol: AP4M1
• NCBI Official Synonym Symbols: MU-4; CPSQ3; SPG50; MU-ARP2
• NCBI Protein Information: AP-4 complex subunit mu-1; mu4; mu4-adaptin; mu subunit of AP-4; mu-adaptin-related protein 2; mu-adaptin-related protein-2; AP-4 adaptor complex mu subunit; adapter-related protein complex 4 mu-1 subunit; adapter-related protein complex 4 subunit mu-1; adaptor-related protein complex 4 subunit mu-1; adaptor-related protein complex AP-4 mu4 subunit
• UniProt Protein Name: AP-4 complex subunit mu-1
• Protein Family: AP-4 complex
• UniProt Gene Name: AP4M1
• UniProt Synonym Gene Names: MUARP2; mu-ARP2; mu4
• UniProt Entry Name: AP4M1_HUMAN

NCBI Description

This gene encodes a subunit of the heterotetrameric AP-4 complex. The encoded protein belongs to the adaptor complexes medium subunits family. This AP-4 complex is involved in the recognition and sorting of cargo proteins with tyrosine-based motifs from the trans-golgi network to the endosomal-lysosomal system. [provided by RefSeq, Jul 2008]

Uniprot Description

AP4M1: Subunit of novel type of clathrin- or non-clathrin- associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. Defects in AP4M1 are the cause of cerebral palsy spastic quadriplegic type 3 (CPSQ3). A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals present postnatally with early infantile hypotonia, delayed psychomotor development, strabismus, lack of independent walking and severe mental retardation. They develop progressive spasticity of all limbs with generalized hypertonia, hyperreflexia, and extensor plantar responses by the end of the first year of life. Speech is absent or limited. Pseudobulbar signs, such as drooling, stereotypic laughter, and exaggerated jaw jerk, are part of the clinical picture. Belongs to the adaptor complexes medium subunit family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: clathrin adaptor complex; Golgi trans cisterna; AP-type membrane coat adaptor complex; coated pit; trans-Golgi network

Molecular Function: transporter activity

Biological Process: vesicle-mediated transport; intracellular protein transport; Golgi to endosome transport

Disease: Spastic Paraplegia 50, Autosomal Recessive