Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit | ANKRD1 elisa kit

Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit

Cat. Num. AAA452156

• Gene Names: Ankrd1; Alrp; CARP; Crap; MARP1
• Reactivity: Mouse
• Synonyms: Ankyrin Repeat Domain Protein 1 (ANKRD1); Mouse Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit; CARP; ALRP; C-193; CVARP; MCARP; Ankyrin Repeat Domain 1(Cardiac Muscle); Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193; Cytokine-inducible nuclear protein; ANKRD1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANKRD1. No significant cross-reactivity or interference between ANKRD1 and analogues was observed.
• Sequence Length: 319

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.052ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANKRD1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANKRD1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANKRD1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANKRD1 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANKRD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ANKRD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANKRD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ANKRD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31560506
• NCBI GeneID: 107765
• NCBI Accession #: NP_038496.2
• NCBI GenBank Nucleotide #: NM_013468.3
• UniProt Accession #: Q9CR42; O55014; Q3UIF7; Q3UJ39; Q792Q9
• Molecular Weight: 36,004 Da
• NCBI Official Full Name: ankyrin repeat domain-containing protein 1
• NCBI Official Synonym Full Names: ankyrin repeat domain 1 (cardiac muscle)
• NCBI Official Symbol: Ankrd1
• NCBI Official Synonym Symbols: Alrp; CARP; Crap; MARP1
• NCBI Protein Information: ankyrin repeat domain-containing protein 1
• UniProt Protein Name: Ankyrin repeat domain-containing protein 1
• Protein Family: Ankyrin repeat domain-containing protein
• UniProt Gene Name: Ankrd1
• UniProt Synonym Gene Names: Carp

Uniprot Description

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes.