Typical Testing Data/Standard Curve (for reference only)
Mouse ACSL1/Long-Chain-Fatty-Acid--CoA Ligase 1 ELISA Kit | ACSL1 elisa kit
Mouse ACSL1/Long-Chain-Fatty-Acid--CoA Ligase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to a family of acyl coenzyme A synthetase proteins, which convert long chain fatty acids to acyl CoA products via an ATP-dependent pathway. This enzyme is enriched in heart, liver and adipose tissue, where it functions in lipid synthesis and mitochondrial and peroxisomal beta-oxidation. In addition, it is expressed in monocytes and macrophages where it appears to have a functionally distinct role in mediating inflammatory and innate immune responses. A pseudogene of this gene is found on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Uniprot Description
ACSL1: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Membrane protein, integral; Lipid Metabolism - fatty acid; EC 6.2.1.3
Cellular Component: peroxisomal membrane; mitochondrial outer membrane; mitochondrion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; plasma membrane; peroxisome
Molecular Function: acetate-CoA ligase (ADP-forming) activity; nucleotide binding; catalytic activity; ATP binding; ligase activity; long-chain-fatty-acid-CoA ligase activity
Biological Process: response to organic substance; triacylglycerol metabolic process; metabolic process; lipid biosynthetic process; adiponectin-mediated signaling pathway; lipid metabolic process; long-chain fatty acid metabolic process; fatty acid metabolic process; fatty acid transport; xenobiotic catabolic process
Research Articles on ACSL1
Similar Products
Product Notes
The Mouse ACSL1 acsl1 (Catalog #AAA2890508) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2890508 ELISA Kit recognizes Mouse ACSL1. It is sometimes possible for the material contained within the vial of "ACSL1/Long-Chain-Fatty-Acid--CoA Ligase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
