Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse A-kinase anchor protein SPHKAP (SPHKAP) Standard Curve.)
Mouse A-Kinase Anchor Protein SPHKAP (SPHKAP) ELISA Kit | SPHKAP elisa kit
Mouse A-Kinase Anchor Protein SPHKAP (SPHKAP) ELISA Kit
Gene Names
Sphkap; SKIP; AI852220; mKIAA1678; 4930544G21Rik; A930009L15Rik
Reactivity
Mouse
Synonyms
A-Kinase Anchor Protein SPHKAP (SPHKAP); Mouse A-Kinase Anchor Protein SPHKAP (SPHKAP) ELISA Kit; SPHKAP; DKFZp781H143; DKFZp781J171; KIAA1678; MGC132614; MGC132616; SKIP; SPHK1 (sphingosine kinase type 1) interacting protein; sphingosine kinase type 1-interacting protein; SPHKAP elisa kit
Reactivity
Mouse
Specificity
Mouse A-kinase anchor protein SPHKAP (SPHKAP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPHKAP. No significant cross-reactivity or interference between Mouse SPHKAP and analogues was observed.
Sequence Length
1658
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse A-kinase anchor protein SPHKAP (SPHKAP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse A-kinase anchor protein SPHKAP (SPHKAP) Standard Curve.)
Related Product Information for SPHKAP elisa kit
Description: This Mouse A-kinase anchor protein SPHKAP (SPHKAP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHKAP in samples. An antibody specific for SPHKAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPHKAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHKAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHKAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPHKAP (SPHK1 Interactor, AKAP Domain Containing) is a Protein Coding gene. GO annotations related to this gene include protein kinase A binding. An important paralog of this gene is AKAP11. Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.
Background: SPHKAP (SPHK1 Interactor, AKAP Domain Containing) is a Protein Coding gene. GO annotations related to this gene include protein kinase A binding. An important paralog of this gene is AKAP11. Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
A-kinase anchor protein SPHKAP isoform 2
NCBI Official Synonym Full Names
SPHK1 interactor, AKAP domain containing
NCBI Official Symbol
Sphkap
NCBI Official Synonym Symbols
SKIP; AI852220; mKIAA1678; 4930544G21Rik; A930009L15Rik
NCBI Protein Information
A-kinase anchor protein SPHKAP
UniProt Protein Name
A-kinase anchor protein SPHKAP
Protein Family
UniProt Gene Name
Sphkap
UniProt Synonym Gene Names
Kiaa1678
UniProt Entry Name
SPKAP_MOUSE
Research Articles on SPHKAP
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Product Notes
The Mouse SPHKAP sphkap (Catalog #AAA9716990) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716990 ELISA Kit recognizes Mouse SPHKAP. It is sometimes possible for the material contained within the vial of "A-Kinase Anchor Protein SPHKAP (SPHKAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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