Typical Testing Data/Standard Curve (for reference only)
Mouse 5, 6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1) ELISA Kit | TYRP1 elisa kit
Mouse 5, 6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TYRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TYRP1 antibody and an TYRP1-HRP conjugate. The assay sample and buffer are incubated together with TYRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TYRP1 concentration since TYRP1 from samples and TYRP1-HRP conjugate compete for the anti-TYRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TYRP1 from the sample, fewer sites are left to bind TYRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TYRP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]
Uniprot Description
TYRP1: Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3); also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. Belongs to the tyrosinase family.
Protein type: EC 1.14.18.-; Oxidoreductase; Amino Acid Metabolism - tyrosine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p23
Cellular Component: melanosome membrane; integral to membrane; melanosome; endosome membrane
Molecular Function: protein binding; protein homodimerization activity; copper ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen; protein heterodimerization activity
Biological Process: melanin biosynthetic process; melanosome organization and biogenesis; melanocyte differentiation; acetoacetic acid metabolic process
Disease: Albinism, Oculocutaneous, Type Iii; Skin/hair/eye Pigmentation, Variation In, 11
Research Articles on TYRP1
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Product Notes
The Mouse TYRP1 tyrp1 (Catalog #AAA7243063) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7243063 ELISA Kit recognizes Mouse TYRP1. It is sometimes possible for the material contained within the vial of "5, 6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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