Mouse Nitric Oxide Synthase 2, Inducible (NOS2) DIY ELISA Kit | NOS2 diy elisa kit

Mouse Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit DIY Materials

Cat. Num. AAA2088859

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Mouse
• Synonyms: Nitric Oxide Synthase 2; Inducible (NOS2); Mouse Nitric Oxide Synthase 2; Inducible (NOS2) ELISA Kit DIY Materials; NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2 diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2). No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.
• Sequence Length: 1153

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.116ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for NOS2 diy elisa kit

Enzyme & Kinase; Metabolic pathway; Tumor immunity; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 126,749 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; intracellular; nucleus; peroxisome

Molecular Function: FAD binding; FMN binding; heme binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; receptor binding

Biological Process: arginine catabolic process; cell redox homeostasis; defense response to bacterium; negative regulation of blood pressure; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of leukocyte mediated cytotoxicity; positive regulation of vasodilation; prostaglandin secretion; regulation of cellular respiration; regulation of insulin secretion; superoxide metabolic process

Disease: Hypertension, Essential; Malaria, Susceptibility To