Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) DIY ELISA Kit | FABP2 diy elisa kit

Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit DIY Materials

Cat. Num. AAA2088656

• Gene Names: FABP2; FABPI; I-FABP
• Reactivity: Mouse
• Synonyms: Fatty Acid Binding Protein 2; Intestinal (FABP2); Mouse Fatty Acid Binding Protein 2; Intestinal (FABP2) ELISA Kit DIY Materials; FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2 diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 2, Intestinal (FABP2). No significant cross-reactivity or interference between Fatty Acid Binding Protein 2, Intestinal (FABP2) and analogues was observed.
• Sequence Length: 132

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for FABP2 diy elisa kit

Metabolic pathway; Tumor immunity; Endocrinology; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 119594072
• NCBI GeneID: 2169
• Molecular Weight: 15,207 Da
• NCBI Official Full Name: fatty acid binding protein 2, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2
• NCBI Official Symbol: FABP2
• NCBI Official Synonym Symbols: FABPI; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal
• UniProt Protein Name: Fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP2
• UniProt Synonym Gene Names: FABPI; I-FABP

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP2: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding; Transporter

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: digestion; triacylglycerol catabolic process