Mouse Coagulation Factor XIII A1 Polypeptide (F13A1) DIY ELISA Kit | F13A1 diy elisa kit

Mouse Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit DIY Materials

Cat. Num. AAA2089012

• Gene Names: F13A1; F13A
• Reactivity: Mouse
• Synonyms: Coagulation Factor XIII A1 Polypeptide (F13A1); Mouse Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit DIY Materials; F13-A1; FXIIIA1; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain; Coagulation factor XIII A chain; F13A1 diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Coagulation Factor XIII A1 Polypeptide (F13A1). No significant cross-reactivity or interference between Coagulation Factor XIII A1 Polypeptide (F13A1) and analogues was observed.
• Sequence Length: 732

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.70ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for F13A1 diy elisa kit

Signal transduction; Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 119395709
• NCBI GeneID: 2162
• NCBI Accession #: NP_000120.2
• NCBI GenBank Nucleotide #: NM_000129.3
• UniProt Accession #: P00488; Q59HA7; Q8N6X2; Q96P24; Q9BX29
• Molecular Weight: 83,267 Da
• NCBI Official Full Name: coagulation factor XIII A chain
• NCBI Official Synonym Full Names: coagulation factor XIII A chain
• NCBI Official Symbol: F13A1
• NCBI Official Synonym Symbols: F13A
• NCBI Protein Information: coagulation factor XIII A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• Protein Family: Coagulation factor
• UniProt Gene Name: F13A1
• UniProt Synonym Gene Names: F13A; Coagulation factor XIIIa

NCBI Description

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: extracellular region

Molecular Function: protein-glutamine gamma-glutamyltransferase activity

Biological Process: blood coagulation; peptide cross-linking; platelet degranulation

Disease: Factor Xiii, A Subunit, Deficiency Of; Myocardial Infarction, Susceptibility To; Thrombophilia Due To Thrombin Defect