Mouse Alpha-1-Antitrypsin (a1AT) DIY ELISA Kit | a1AT diy elisa kit
Mouse Alpha-1-Antitrypsin (a1AT) ELISA Kit DIY Materials
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Mouse
Synonyms
Alpha-1-Antitrypsin (a1AT); Mouse Alpha-1-Antitrypsin (a1AT) ELISA Kit DIY Materials; SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor; Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1; a1AT diy elisa kit
Reactivity
Mouse
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
Sequence Length
418
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.2-2000ng/mL
Sensitivity
12.8ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for a1AT diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,755 Da
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin family A member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
NCBI Description
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Research Articles on a1AT
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Product Notes
The Mouse a1AT serpina1 (Catalog #AAA2089139) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089139 DIY ELISA Kit recognizes Mouse a1AT. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the a1AT serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-1-Antitrypsin (a1AT), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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