Typical Testing Data/Standard Curve (for reference only)
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit | RANTES clia kit
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to RANTES. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of RANTES. You can calculate the concentration of RANTES in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Chemokine; Secreted; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: extracellular space; cytoplasm; extracellular region
Molecular Function: heparin binding; protein homodimerization activity; protein self-association; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; CCR4 chemokine receptor binding; CCR1 chemokine receptor binding; protein binding; chemokine receptor binding; chemokine activity; chemokine receptor antagonist activity; CCR5 chemokine receptor binding; chemoattractant activity; phospholipase activator activity
Biological Process: regulation of chronic inflammatory response; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; response to toxin; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of homotypic cell-cell adhesion; monocyte chemotaxis; leukocyte adhesion; positive chemotaxis; cell-cell signaling; positive regulation of T cell proliferation; response to drug; neutrophil chemotaxis; positive regulation of viral genome replication; neutrophil activation; response to virus; positive regulation of cellular biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; response to estrogen stimulus; eosinophil chemotaxis; response to activity; positive regulation of epithelial cell proliferation; regulation of T cell activation; exocytosis; dibenzo-p-dioxin metabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; chemotaxis; positive regulation of cell-cell adhesion mediated by integrin; response to salt stress; response to insulin stimulus; positive regulation of translational initiation; calcium ion transport; positive regulation of innate immune response; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; dendritic cell chemotaxis; inflammatory response; lymphocyte chemotaxis; protein tetramerization; aging; phospholipase D activation; chronic inflammatory response; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; macrophage chemotaxis; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; cellular protein complex assembly; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of viral genome replication; positive regulation of fever; positive regulation of calcium ion transport; positive regulation of neuron differentiation; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of GTPase activity; positive regulation of cell migration; positive regulation of inflammatory response
Research Articles on RANTES
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Product Notes
The Mouse RANTES ccl5 (Catalog #AAA2530657) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530657 CLIA Kit recognizes Mouse RANTES. It is sometimes possible for the material contained within the vial of "RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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