Mouse Heat Shock Protein 60 (Hsp60) CLIA Kit | Hsp60 clia kit

Heat Shock Protein 60 (Hsp60) CLIA Kit

Cat. Num. AAA2709433

• Gene Names: Hspd1; 60kDa; CPN60; Hsp60; HSP-60; HSP-65
• Reactivity: Mouse
• Applications: Chemiluminescent Immunoassay (CLIA)
• Synonyms: Heat Shock Protein 60 (Hsp60); Heat Shock Protein 60 (Hsp60) CLIA Kit; HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1; Chaperonin; Spastic Paraplegia 13; Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein; Hsp60 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hsp60. No significant cross-reactivity or interference between Hsp60 and analogues was observed.

• Applicable Applications for Hsp60 clia kit: Chemiluminescent Immunoassay (CLIA)
• Application Notes: Chemiluminescent immunoassay for Antigen Detection.
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.14-100ng/mL
• Sensitivity: < 0.04ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hsp60 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hsp60 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hsp60 clia kit

Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of Hsp60 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Hsp60. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hsp60. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hsp60 level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 183396771
• NCBI GeneID: 15510
• NCBI Accession #: NP_034607.3
• NCBI GenBank Nucleotide #: NM_010477.4
• UniProt Accession #: P63038; P19226; P19227; P97602; Q3KQP2; Q3UIP0; Q8C2C7; Q8VEF4
• Molecular Weight: 27,015 Da
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein 1 (chaperonin)
• NCBI Official Symbol: Hspd1
• NCBI Official Synonym Symbols: 60kDa; CPN60; Hsp60; HSP-60; HSP-65
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• Protein Family: Hydrogenosomal chaperonin
• UniProt Gene Name: Hspd1
• UniProt Synonym Gene Names: Hsp60; CPN60; HSP-60; Hsp60

Uniprot Description

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.