Mouse Granulin (GRN) CLIA Kit | GRN clia kit

Mouse Granulin (GRN) CLIA Kit

Cat. Num. AAA2001165

• Gene Names: GRN; GEP; GP88; PEPI; PGRN; CLN11; PCDGF
• Reactivity: Mouse
• Synonyms: Granulin (GRN); Mouse Granulin (GRN) CLIA Kit; GEP; GP88; PCDGF; PEPI; PGRN; Proepithelin; Acrogranin; Glycoprotein of 88 Kda; Paragranulin; GRN clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Granulin (GRN).; No significant cross-reactivity or interference between Granulin (GRN) and analogues was observed.
• Sequence Length: 593

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.14-100ng/mL
• Sensitivity: < 0.05ng/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulin (GRN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulin (GRN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRN clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Granulin (GRN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Granulin (GRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Granulin (GRN) level in the sample or standard.;

Product Categories/Family for GRN clia kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 183613
• NCBI GeneID: 2896
• UniProt Accession #: P28799; P23781; P23782; P23783; P23784; Q53HQ8; Q53Y88; Q540U8; Q9BWE7; Q9H8S1; Q9UCH0; D3DX55
• Molecular Weight: 44,132 Da
• NCBI Official Full Name: granulin
• NCBI Official Synonym Full Names: granulin
• NCBI Official Symbol: GRN
• NCBI Official Synonym Symbols: GEP; GP88; PEPI; PGRN; CLN11; PCDGF
• NCBI Protein Information: granulins
• UniProt Protein Name: Granulins
• Protein Family: Granulin
• UniProt Gene Name: GRN
• UniProt Synonym Gene Names: PEPI; GP88; Glycoprotein 88
• UniProt Entry Name: GRN_HUMAN

NCBI Description

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GRN: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD); also known as tau- negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Defects in GRN are the cause of neuronal ceroid lipofuscinosis type 11 (CLN11). A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material. Belongs to the granulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle

Molecular Function: protein binding; growth factor activity; cytokine activity

Biological Process: blastocyst hatching; signal transduction; positive regulation of epithelial cell proliferation; embryo implantation

Disease: Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related; Ceroid Lipofuscinosis, Neuronal, 11