Mouse Cerberus (CER) CLIA Kit | CER clia kit

Mouse Cerberus (CER) CLIA Kit

Cat. Num. AAA2709073

• Gene Names: Cer1; Cerl; Cerl1; Cerr1; cer-1
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Cerberus (CER); Mouse Cerberus (CER) CLIA Kit; Cerberus; CER1; DAND4; Cerberus 1; DAN domain family member 4; Cysteine Knot Superfamily; Homolog(Xenopus Laevis); Cerberus-related protein; CER clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cerberus (CER).; No significant cross-reactivity or interference between Cerberus (CER) and analogues was observed.
• Sequence Length: 272

• Applicable Applications for CER clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.96pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerberus (CER) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerberus (CER) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cerberus (CER). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cerberus (CER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cerberus (CER) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6753410
• NCBI GeneID: 12622
• NCBI Accession #: NP_034017.1
• NCBI GenBank Nucleotide #: NP_034017.1
• UniProt Accession #: O55233
• NCBI Official Full Name: cerberus
• NCBI Official Synonym Full Names: cerberus 1, DAN family BMP antagonist
• NCBI Official Symbol: Cer1
• NCBI Official Synonym Symbols: Cerl; Cerl1; Cerr1; cer-1
• NCBI Protein Information: cerberus
• UniProt Protein Name: Cerberus
• UniProt Gene Name: Cer1
• UniProt Synonym Gene Names: Cerl; Cerr1; Cer-l

Uniprot Description

Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak.