Trem2 blocking peptide

Mouse Trem2 Antibody (N-term) Blocking Peptide

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Synonyms: Trem2; Mouse Trem2 Antibody (N-term) Blocking Peptide; Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; Trem2a; Trem2b; Trem2c; Trem2 blocking peptide

• Host: Mouse
• Form/Format: Peptide was lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropritate buffer as needed.
• Sequence Length: 227

• Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Trem2 blocking peptide

May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed:27477018, PubMed:29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed:28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed:15728241, PubMed:28592261, PubMed:29752066, PubMed:25631124). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed:30232263, PubMed:29663649, PubMed:28483841, PubMed:29859094). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed:29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed:28592261). Plays a role in microglial MTOR activation and metabolism (PubMed:28802038). Regulates age-related changes in microglial numbers (PubMed:30548312, PubMed:29752066, PubMed:25631124). Triggers activation of the immune responses in macrophages and dendritic cells (By similarity). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). In dendritic cells, it mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (By similarity). Involved in the positive regulation of osteoclast differentiation (PubMed:16418779).

NCBI and Uniprot Product Information

• NCBI GI #: 50401660
• NCBI GeneID: 83433
• UniProt Accession #: Q99NH8; Q8CGK4; Q8CIA6; Q99NH9; Q9JL34
• NCBI Official Full Name: Triggering receptor expressed on myeloid cells 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem2
• UniProt Synonym Gene Names: Trem2a; Trem2b; Trem2c; TREM-2
• UniProt Entry Name: TREM2_MOUSE

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: integral to membrane; intracellular membrane-bound organelle

Molecular Function: lipopolysaccharide binding; peptidoglycan binding; protein binding; transmembrane receptor activity

Biological Process: detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; lipopolysaccharide-mediated signaling pathway; phagocytosis, engulfment; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation