S100 Calcium Binding Protein A13 Active Protein | S100A13 active protein

S100 Calcium Binding Protein A13, Recombinant, Mouse (S100A13)

Cat. Num. AAA636535

• Reactivity: Mouse
• Purity: Purified; > 97%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: S100 Calcium Binding Protein A13; Recombinant; Mouse (S100A13); S100A13 active protein

• Reactivity: Mouse
• Purity/Purification: Purified; > 97%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to a concentration of >100ug/ml. For long term storage a carrier protein (e.g. 0.1% HSA or BSA) should be added.

• Biological Activity: Measured by its ability to enhance neurite outgrowth of cortical neurons from E18 rat embryos. Immobilized rmS100A13 (at a 3il droplet containing 50 ng) on a nitrocellulose coated microplate is sufficient to significantly enhance neurite outgrowth.
• Endotoxin Level: 1EU/ug of the protein as determined by the LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 1 month at 4 degree C and 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for S100A13 active protein

Mouse S100A13 is an 11kD member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca ++ -binding proteins. It is widely expressed as a homodimer with 98 amino acid (aa) long subunits. Mouse S100A13 shares 87%, 83%, 91%, 86%, 81% and 53% aa identity with rat, human, cow, dog, opossum and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but it contains a basic residue-rich sequence at the C terminus, and two EF hand motifs that bind Ca++ with differing affinities. Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation end-products (RAGE).

Product Categories/Family for S100A13 active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47123484
• NCBI GeneID: 6284
• UniProt Accession #: Q99584; Q52PI9; Q6FGF8
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: S100 calcium binding protein A13
• NCBI Official Synonym Full Names: S100 calcium binding protein A13
• NCBI Official Symbol: S100A13
• NCBI Protein Information: protein S100-A13; S100 calcium-binding protein A13
• UniProt Protein Name: Protein S100-A13
• Protein Family: Protein
• UniProt Gene Name: S100A13
• UniProt Entry Name: S10AD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A13: Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; RAGE receptor binding; protein homodimerization activity; fibroblast growth factor binding; copper ion binding; zinc ion binding; calcium ion binding; lipid binding

Biological Process: regulation of cell shape; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to copper ion; interleukin-1 alpha secretion; positive regulation of cell proliferation; mast cell degranulation; response to electrical stimulus; cytokine secretion