Elastase, Neutrophil Active Protein
Elastase, Neutrophil, Recombinant, Mouse
Reactivity
Mouse
Purity
Highly Purified~ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0EU/ug (LAL).
Synonyms
Elastase; Neutrophil; Recombinant; Mouse; Neutrophil active protein
Reactivity
Mouse
Purity/Purification
Highly Purified
~ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0EU/ug (LAL).
~ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0EU/ug (LAL).
Form/Format
Supplied as a liquid in 25mM Tris, 1M sodium chloride, pH 7.5.
Concentration
0.44mg/ml (varies by lot)
Application Notes
SDS-PAGE: 34kD, Reducing conditions
Specific Activity
~ 500pmoles/min/ug. Measured by its ability to cleave a fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC).
Endotxin
<1 EU/ug (LAL)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Elastase, Neutrophil active protein
Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function is the degradation of many extracellular matrix macromolecules. alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. This protein may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases. The purified recombinant mouse ELA2 does not contain the last five amino acid residues of the deduced amino acid sequence.
Murine myeloma cell line, NSO-derived Ser27-Arg260 with a C-terminallO-His tag.
Murine myeloma cell line, NSO-derived Ser27-Arg260 with a C-terminallO-His tag.
Product Categories/Family for Elastase, Neutrophil active protein
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Product Notes
The Elastase, Neutrophil (Catalog #AAA637582) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Elastase, Neutrophil, Recombinant, Mouse reacts with Mouse and may cross-react with other species as described in the data sheet. SDS-PAGE: 34kD, Reducing conditions. Researchers should empirically determine the suitability of the Elastase, Neutrophil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastase, Neutrophil, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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