Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. ZFP64 Mouse Monoclonal Antibody (PCRP-ZFP64-1H2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Mouse anti-Human ZFP64 Monoclonal Antibody | anti-ZFP64 antibody
ZFP64 Mouse Monoclonal Antibody [Clone PCRP-ZFP64-1H2]
Gene Names
ZFP64; ZNF338
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
ZFP64; Monoclonal Antibody; ZFP64 Mouse Monoclonal Antibody [Clone PCRP-ZFP64-1H2]; dJ548G19. 1; dJ831D17. 1; ZFP64 zinc finger protein; Zinc finger protein 338; Zinc finger protein 64; Zinc finger protein 64 homolog; Zinc finger protein 64 isoforms 1 and 2; Zinc finger protein 64 isoforms 3 and 4; ZNF338; anti-ZFP64 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG2b
Clone Number
PCRP-ZFP64-1H2
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-ZFP64 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
Immunogen
Recombinant full-length human ZFP64 protein
Cellular Localization
Nucleus, Cytoplasm, Cell surface
Positive Control
HeLa or HepG2 cells.
Hu-chromosome Location
20q13.2
Unigene
473082
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. ZFP64 Mouse Monoclonal Antibody (PCRP-ZFP64-1H2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. ZFP64 Mouse Monoclonal Antibody (PCRP-ZFP64-1H2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Related Product Information for anti-ZFP64 antibody
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZFP64 (Zinc finger protein 64), also known as ZNF338, is a 681 amino acid homolog of the mouse Zfp64 protein and is a member of the Kr ppel C2H2-type zinc-finger family. Localized to the nucleus, ZFP64 contains nine C2H2-type zinc fingers and is thought to be involved in transcriptional regulation. Four isoforms of ZFP64 exist due to alternative splicing events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,463 Da
NCBI Official Full Name
zinc finger protein 64 isoform e
NCBI Official Synonym Full Names
ZFP64 zinc finger protein
NCBI Official Symbol
ZFP64
NCBI Official Synonym Symbols
ZNF338
NCBI Protein Information
zinc finger protein 64
UniProt Protein Name
Zinc finger protein 64 homolog, isoforms 3 and 4
Protein Family
UniProt Gene Name
ZFP64
UniProt Synonym Gene Names
ZNF338; Zfp-64
Uniprot Description
May be involved in transcriptional regulation.
Research Articles on ZFP64
1. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
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Product Notes
The ZFP64 zfp64 (Catalog #AAA4382148) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZFP64 Mouse Monoclonal Antibody [Clone PCRP-ZFP64-1H2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZFP64 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ZFP64 zfp64 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFP64, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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