Mouse anti-Human, Rat ZAP70 Monoclonal Antibody | anti-ZAP70 antibody

Mouse Monoclonal [clone 11H40] to Human ZAP70

Cat. Num. AAA245383

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: ZAP70; Monoclonal Antibody; Mouse Monoclonal [clone 11H40] to Human ZAP70; Anti-ZAP70 Antibody (clone 11H40) IHC-plus; 70 kDa zeta-associated protein; SRK; STCD; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; Zeta-associated protein 70; Human ZAP70; anti-ZAP70 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Clone Number: 11H40
• Specificity: Recognizes human ZAP-70.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.2, 0.09% azide, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 619

• Applicable Applications for anti-ZAP70 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (20 ug/ml), WB (1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant full length human Zap-70.
• Immunogen Type: Recombinant protein
• Immunogen: ZAP70 antibody was raised against recombinant full length human Zap-70.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ZAP70 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Western Blot (WB)

(Lane 1: MW marker, Lane 2: Jurkat, Lane 3: rat thymus, Lane 4: human recombinant GST tagged Zap-70.)

Related Product Information for anti-ZAP70 antibody

ZAP70 is a tyrosine kinase that associates with the T-cell antigen receptor zeta chain and mediates lymphocyte activation. Defects in ZAP70 cause immunodeficiency diseases such as selective T-cell defect.

Product Categories/Family for anti-ZAP70 antibody

Family: Protein Kinase; Subfamily: Syk/ZAP70

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 55,873 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; zeta-chain associated protein kinase, 70kD
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: adaptive immune response; cell migration; peptidyl-tyrosine phosphorylation; T cell activation; B cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of T cell differentiation; innate immune response; immune response; beta selection; positive regulation of alpha-beta T cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; T cell differentiation; positive thymic T cell selection

Disease: Selective T-cell Defect