Western Blot (WB)
(WB: 0.5-1μg/ml ofZ0300-03D detected Zap-70 in RIPA lysates from Jurkat cells.)
Mouse anti-Human, Mouse ZAP 70 Monoclonal Antibody | anti-ZAP 70 antibody
ZAP 70 (TCR Zeta Associated Protein kinase 70kD) (Biotin)
Western Blot (WB)
(WB: 0.5-1μg/ml ofZ0300-03D detected Zap-70 in RIPA lysates from Jurkat cells.)
Western Blot (WB)
(WB: 0.5-1μg/ml ofZ0300-03D detected Zap-70 in RIPA lysates from Jurkat cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft
Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding
Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; immune response; positive regulation of alpha-beta T cell proliferation; beta selection; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; positive thymic T cell selection
Disease: Selective T-cell Defect
Research Articles on ZAP 70
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Product Notes
The ZAP 70 zap70 (Catalog #AAA6242936) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZAP 70 (TCR Zeta Associated Protein kinase 70kD) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP 70 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the ZAP 70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAP 70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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