Mouse WWTR1 Monoclonal Antibody | anti-WWTR1 antibody

WWTR1 antibody

Cat. Num. AAA834947

• Gene Names: WWTR1; TAZ
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: WWTR1 antibody was purified by affinity chromatography.
• Synonyms: WWTR1; Monoclonal Antibody; WWTR1 antibody; Monoclonal WWTR1; Anti-WWTR1; WW domain containing transcription regulator 1; TAZ; DKFZp586I1419; Transcriptional coactivator with PDZ-binding motif; FLJ45718; WW domain-containing transcription regulator protein 1; WWTR 1; WWTR-1; anti-WWTR1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1B5
• Purity/Purification: WWTR1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-WWTR1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:200-500
• Biological Significance: WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylatesand in activates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation.
• Cross-Reactivity: Human
• Immunogen: WWTR1 antibody was raised in mouse using a full length recombinant protein of human WWTR1 (NP_056287) produced in HEK293 cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant WWTR1 protein (Right) or empty vector (Left) detected with WWTR1 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant WWTR1 protein using WWTR1 antibody)

Related Product Information for anti-WWTR1 antibody

Mouse monoclonal WWTR1 antibody

Product Categories/Family for anti-WWTR1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13346498
• NCBI GeneID: 25937
• NCBI Accession #: NP_056287
• NCBI GenBank Nucleotide #: NM_015472.4
• UniProt Accession #: Q9GZV5; Q8N3P2; Q9Y3W6; D3DNH7
• Molecular Weight: 44,101 Da
• NCBI Official Full Name: WW domain-containing transcription regulator protein 1
• NCBI Official Synonym Full Names: WW domain containing transcription regulator 1
• NCBI Official Symbol: WWTR1
• NCBI Official Synonym Symbols: TAZ
• NCBI Protein Information: WW domain-containing transcription regulator protein 1
• UniProt Protein Name: WW domain-containing transcription regulator protein 1
• Protein Family: WW domain-containing transcription regulator protein
• UniProt Gene Name: WWTR1
• UniProt Synonym Gene Names: TAZ
• UniProt Entry Name: WWTR1_HUMAN

Uniprot Description

TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.

Protein type: Transcription, coactivator/corepressor; Oncoprotein

Chromosomal Location of Human Ortholog: 3q23-q24

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; transcription coactivator activity; transcription corepressor activity

Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; multicellular organism growth; stem cell division; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; glomerulus development; osteoblast differentiation; mesenchymal cell differentiation; negative regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; gene expression