Mouse anti-Human WRN Monoclonal Antibody | anti-WRN antibody

WRN (Werner Syndrome Helicase, Werner's Syndrome Helicase WRN, Werner Syndrome Protein, HGNC:12791, RECQ3, RECQL2, RECQL3) (Azide Free)

Cat. Num. AAA631445

• Gene Names: WRN; RECQ3; RECQL2; RECQL3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: WRN; Monoclonal Antibody; WRN (Werner Syndrome Helicase; Werner's Syndrome Helicase WRN; Werner Syndrome Protein; HGNC:12791; RECQ3; RECQL2; RECQL3) (Azide Free); Anti -WRN (Werner Syndrome Helicase; anti-WRN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 11C960
• Specificity: Recognizes human WRN.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

• Applicable Applications for anti-WRN antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Immunofluorescence, and Western Blot.; Dilution: ELISA: 1ug/ml-3ng/ml; Western Blot: 1:500-1:1,000
• Immunogen: Recombinant protein corresponding to aa1322-1433 of human WRN.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-WRN antibody

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq]

Product Categories/Family for anti-WRN antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 110735439
• NCBI GeneID: 7486
• NCBI Accession #: NP_000544.2
• NCBI GenBank Nucleotide #: NM_000553.4
• UniProt Accession #: Q14191; A1KYY9
• Molecular Weight: 162,461 Da
• NCBI Official Full Name: Werner syndrome ATP-dependent helicase
• NCBI Official Synonym Full Names: Werner syndrome, RecQ helicase-like
• NCBI Official Symbol: WRN
• NCBI Official Synonym Symbols: RECQ3; RECQL2; RECQL3
• NCBI Protein Information: Werner syndrome ATP-dependent helicase; exonuclease WRN; recQ protein-like 2; DNA helicase, RecQ-like type 3
• UniProt Protein Name: Werner syndrome ATP-dependent helicase
• Protein Family: Werner syndrome ATP-dependent helicase
• UniProt Gene Name: WRN
• UniProt Synonym Gene Names: RECQ3; RECQL2; RecQ3
• UniProt Entry Name: WRN_HUMAN

NCBI Description

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]

Uniprot Description

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: Nucleolus; DNA repair, damage; DNA-binding; Cell cycle regulation; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: MutLalpha complex; nucleoplasm; centrosome; nucleolus

Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; Y-form DNA binding; ATP-dependent DNA helicase activity; four-way junction helicase activity; protein binding; DNA helicase activity; DNA binding; ATP-dependent 3'-5' DNA helicase activity; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding

Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; cell aging; multicellular organismal aging; replicative cell aging; cellular response to starvation; DNA duplex unwinding; response to UV-C; DNA recombination; replication fork processing; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; response to oxidative stress; regulation of growth rate; DNA replication; DNA metabolic process; telomere maintenance; response to DNA damage stimulus; aging

Disease: Werner Syndrome