Mouse Wilm's Tumor 1 (WT1) Monoclonal Antibody | anti-WT1 antibody

Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439403

• Gene Names: WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• Reactivity: Human and Rat. Others-not known
• Applications: Immunohistochemistry
• Synonyms: Wilm's Tumor 1 (WT1); Monoclonal Antibody; Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) Mouse Monoclonal Antibody; WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; anti-WT1 antibody

• Host: Mouse
• Reactivity: Human and Rat. Others-not known
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM361
• Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm’s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm’s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the mesotheliomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 497

• Applicable Applications for anti-WT1 antibody: Immunohistology (Formalin-fixed)
• Application Notes: Suggested Dilutions: Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT); Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Immunogen: Recombinant protein corresponding to residues 1-181 of human WT1.
• Cellular Localization: Nuclear and Cytoplasmic
• Positive Control: K562 cells. Wilm’s Tumor, mesothelioma or fetal kidney.
• Supplied As: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.; ; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Fetal Kidney stained with WT1 Monoclonal Antibody (SPM361).)

References

Rauscher JF, Morris JF, Fredericks WJ, Lopez-Guisa J, Balakrishnan C, Jost M, Herlyn M, Rodeck U. Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms; tumor suppressor protein. Hybridoma 1998; 17:191

NCBI and Uniprot Product Information

• NCBI GI #: 65507714
• NCBI GeneID: 7490
• NCBI Accession #: NP_000369.3
• NCBI GenBank Nucleotide #: NM_000378.4
• UniProt Accession #: P19544; Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5
• Molecular Weight: 47-55kDa
• NCBI Official Full Name: Wilms tumor protein isoform A
• NCBI Official Synonym Full Names: Wilms tumor 1
• NCBI Official Symbol: WT1
• NCBI Official Synonym Symbols: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• NCBI Protein Information: Wilms tumor protein
• UniProt Protein Name: Wilms tumor protein
• Protein Family: Wilms tumor protein
• UniProt Gene Name: WT1
• UniProt Entry Name: WT1_HUMAN

NCBI Description

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

Uniprot Description

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).