<
Immunohistochemistry (IHC)
(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse VWA5A Monoclonal Antibody | anti-VWA5A antibody
Anti-VWA5A Antibody (clone 3A3) IHC-plus
Gene Names
VWA5A; BCSC1; BCSC-1; LOH11CR2A
Reactivity
Mouse, Human, Monkey
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein A/G purified
Synonyms
VWA5A; Monoclonal Antibody; Anti-VWA5A Antibody (clone 3A3) IHC-plus; Mouse Monoclonal [clone 3A3] (IgG1) to Human VWA5A; Human VWA5A; BCSC-1; LOH11CR2A; Ortholog of mouse AW551984; BCSC1; anti-VWA5A antibody
Host
Mouse
Reactivity
Mouse, Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A3
Specificity
Human VWA5A
Purity/Purification
Protein A/G purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
786
Applicable Applications for anti-VWA5A antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry (1:100)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:500 - 1:1000)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:500 - 1:1000)
Immunogen
VWA5A antibody was raised against full length human recombinant protein of human VWA5A(NP_055437) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human VWA5A(NP_055437) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Anti-VWA5A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A.)
Immunofluorescence (IF)
(Anti-VWA5A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using g anti-VWA5A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using g anti-VWA5A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody, and then analyzed by flow cytometry.)
Related Product Information for anti-VWA5A antibody
VWA5A Antibody, BCSC-1 Antibody, LOH11CR2A Antibody, Ortholog of mouse AW551984 Antibody, BCSC1 Antibody Description: May play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,235 Da
NCBI Official Full Name
von Willebrand factor A domain-containing protein 5A isoform 1
NCBI Official Synonym Full Names
von Willebrand factor A domain containing 5A
NCBI Official Symbol
VWA5A
NCBI Official Synonym Symbols
BCSC1; BCSC-1; LOH11CR2A
NCBI Protein Information
von Willebrand factor A domain-containing protein 5A
UniProt Protein Name
von Willebrand factor A domain-containing protein 5A
Protein Family
UniProt Gene Name
VWA5A
UniProt Synonym Gene Names
BCSC1; LOH11CR2A; BCSC-1
UniProt Entry Name
VMA5A_HUMAN
Uniprot Description
VWA5A: May play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor
Chromosomal Location of Human Ortholog: 11q24.1
Cellular Component: nucleoplasm; nucleus
Research Articles on VWA5A
Similar Products
Product Notes
The VWA5A vwa5a (Catalog #AAA249350) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-VWA5A Antibody (clone 3A3) IHC-plus reacts with Mouse, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's VWA5A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:100) IF (1:100) IHC-P (10 ug/ml) WB (1:500 - 1:1000). Researchers should empirically determine the suitability of the VWA5A vwa5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VWA5A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
