Mouse VPS35 Monoclonal Antibody | anti-VPS35 antibody

VPS35 Antibody, Clone 5A9: RPE

Cat. Num. AAA8005635

• Gene Names: VPS35; MEM3; PARK17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: VPS35; Monoclonal Antibody; VPS35 Antibody; Clone 5A9: RPE; Mouse Anti-Human VPS35 Monoclonal IgG1; VPS35 Antibody: RPE; Vacuolar protein sorting-associated protein 35; MEM3; PARK17; VPS35 retromer complex component; maternal-embryonic 3; vesicle protein sortin 35; TCCCTA00141; FLJ10752; anti-VPS35 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5A9
• Specificity: Detects ~92 kDa.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 796

• Applicable Applications for anti-VPS35 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC/IF: 1:200; IP: 1:200
• Immunogen Species: Human
• Immunogen: Full length recombinant human VSP35
• Conjugate: RPE
• Cellular Localization: Endosome; Lysosome; Vesicles; Cytoplasm; Membrane
• Tissue Specificity: Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 5A9 depletes VPS35 from the A549 cell extract..)

Immunoprecipitation (IP)

(Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

Western Blot (WB)

(Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

Related Product Information for anti-VPS35 antibody

Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson's Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).

Product Categories/Family for anti-VPS35 antibody

Cell Signaling; Neuroscience |Neurodegeneration; Parkinson's Disease; Membrane Trafficking Proteins; Golgi Proteins; Protein Trafficking; Alzheimer's Disease; Antibodies

References

1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162-167. 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168-175. 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.

NCBI and Uniprot Product Information

• NCBI GI #: 17999541
• NCBI GeneID: 55737
• NCBI Accession #: NP_060676.2
• NCBI GenBank Nucleotide #: NP_060676.2
• UniProt Accession #: Q96QK1
• NCBI Official Full Name: vacuolar protein sorting-associated protein 35
• NCBI Official Synonym Full Names: VPS35 retromer complex component
• NCBI Official Symbol: VPS35
• NCBI Official Synonym Symbols: MEM3; PARK17
• NCBI Protein Information: vacuolar protein sorting-associated protein 35
• UniProt Protein Name: Vacuolar protein sorting-associated protein 35
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS35
• UniProt Synonym Gene Names: MEM3; hVPS35
• UniProt Entry Name: VPS35_HUMAN

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]