Mouse anti-Human VLDL receptor, clone 1H10 Monoclonal Antibody | anti-VLDLR antibody

Anti Human VLDL receptor, clone 1H10

Cat. Num. AAA135058

• Gene Names: VLDLR; CARMQ1; CHRMQ1; VLDLRCH
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: IgG fraction
• Synonyms: VLDL receptor; clone 1H10; Monoclonal Antibody; Anti Human VLDL receptor; anti-VLDLR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 1H10
• Purity/Purification: IgG fraction
• Form/Format: Frozen liquid; 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6
• Concentration: 4.74 mg/ml (varies by lot)
• Sequence Length: 845

• Applicable Applications for anti-VLDLR antibody: ELISA (EIA), Western Blot (WB), Inhibition, Blocking
• Source: Hybridoma cell culture
• Target: Recognizes the C-terminus of VLDL receptor ligand binding domain (191-355)
• Target Molecular Weight: 85,000
• Antigen: Recombinant fragment of soluble VLDL receptor containing ligand repeats 1-8 (sVLDLr1-8).
• Extinction Coefficient: 1.36
• Preparation and Storage: Ships on dry ice. Store at -70°C.

Related Product Information for anti-VLDLR antibody

Monoclonal antibody produced in mouse. Useful for ELISA and western blot under non-reducing conditions. Binds to an epitope on the C-terminus of VLDL receptor ligand binding domain (amino acids 191-355, UniProtKB: locus VLDLR_HUMAN, accession P98155) and blocks apoE4 binding. Purified by immobilized Protein A. IgG1 class.

References:
Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms. Mikhailenko I et al. Journal of Cell Science 112, 3269-3281 (1999).
The apoE isoform binding properties of the VLDL receptor reveal marked differences from LRP and the LDL receptor. Ruiz J et al. Journal of Lipid Research 46, 1721-1731 (2005).

Product Categories/Family for anti-VLDLR antibody

Receptor Associated Protein (RAP); Monoclonal Antibodies to Human VLDL Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 65301164
• NCBI GeneID: 7436
• NCBI Accession #: NP_001018066.1
• NCBI GenBank Nucleotide #: NM_001018056.1
• UniProt Accession #: P98155; Q5VVF6; B2RMZ7; D3DRH6
• Molecular Weight: 160,000
• NCBI Official Full Name: very low-density lipoprotein receptor isoform b
• NCBI Official Synonym Full Names: very low density lipoprotein receptor
• NCBI Official Symbol: VLDLR
• NCBI Official Synonym Symbols: CARMQ1; CHRMQ1; VLDLRCH
• NCBI Protein Information: very low-density lipoprotein receptor; VLDL-R; VLDL receptor
• UniProt Protein Name: Very low-density lipoprotein receptor
• Protein Family: Very low-density lipoprotein receptor
• UniProt Gene Name: VLDLR
• UniProt Synonym Gene Names: VLDL receptor; VLDL-R
• UniProt Entry Name: VLDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: membrane; plasma membrane; integral to membrane; coated pit; receptor complex

Molecular Function: low-density lipoprotein receptor activity; very-low-density lipoprotein receptor activity; very-low-density lipoprotein binding; protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; calcium-dependent protein binding

Biological Process: cholesterol metabolic process; nervous system development; receptor-mediated endocytosis; positive regulation of protein kinase activity; ventral spinal cord development; negative regulation of transcription from RNA polymerase II promoter; lipid transport; signal transduction; memory

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1