Mouse anti-Human VH1-Related Phosphatase, Vaccinia Virus Monoclonal Antibody
VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3)
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
VH1-Related Phosphatase; Vaccinia Virus; Monoclonal Antibody; Vaccinia Virus (VHR; DUSP3); Anti -VH1-Related Phosphatase; anti-VH1-Related Phosphatase; Vaccinia Virus antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8H125
Specificity
Recognizes human VHR.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Applicable Applications for anti-VH1-Related Phosphatase, Vaccinia Virus antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes
Immunohistochemistry, Direct ELISA
Dilution: Immunohistochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect VHR in paraffin-embedded human tonsil tissue sections. For chromogenic detection of labeling, the use of staining kits is recommended.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human VHR. The detection limit for rhVHR is approximately 1 ng/well.
Dilution: Immunohistochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect VHR in paraffin-embedded human tonsil tissue sections. For chromogenic detection of labeling, the use of staining kits is recommended.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human VHR. The detection limit for rhVHR is approximately 1 ng/well.
Immunogen
E. coli-derived rhVHR
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human VHR (rhVHR; aa 2-185; Accession # NM_004090).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-VH1-Related Phosphatase, Vaccinia Virus antibody
Vaccinia Virus VH1-related phosphatase (VHR), also known as dual-specificity phosphatase 3 (DUSP3), dephosphorylates a variety of substrates at serine, threonine, and tyrosine residues.1 VHR has been shown to dephosphorylate the mitogen-activated protein kinases ERK1, ERK2, and JNK, but not p38.2, 3 Phosphorylation of VHR at Y138 by ZAP70 enhances the inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor signaling.3
Product Categories/Family for anti-VH1-Related Phosphatase, Vaccinia Virus antibody
Similar Products
Product Notes
The VH1-Related Phosphatase, Vaccinia Virus (Catalog #AAA601689) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VH1-Related Phosphatase, Vaccinia Virus can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Immunohistochemistry, Direct ELISA Dilution: Immunohistochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect VHR in paraffin-embedded human tonsil tissue sections. For chromogenic detection of labeling, the use of staining kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human VHR. The detection limit for rhVHR is approximately 1 ng/well. Researchers should empirically determine the suitability of the VH1-Related Phosphatase, Vaccinia Virus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VH1-Related Phosphatase, Vaccinia Virus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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