Mouse Verotoxin II-B subunit Monoclonal Antibody
Verotoxin II-B subunit (Shiga-like, SLT-2b)
Applications
ELISA, Neutralization Assay
Purity
Purified by Protein A affinity chromatography from ascites or culture medium (> 90%).
Synonyms
Verotoxin II-B subunit; Monoclonal Antibody; Verotoxin II-B subunit (Shiga-like; SLT-2b); Anti -Verotoxin II-B subunit (Shiga-like; anti-Verotoxin II-B subunit antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
5E458
Specificity
Recognizes Verotoxin II-B subunit (SLT-2b). Neutralizes Verotoxin.
Purity/Purification
Purified by Protein A affinity chromatography from ascites or culture medium (> 90%).
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for anti-Verotoxin II-B subunit antibody
ELISA (EL/EIA), Neutralization
Application Notes
Dilution: ELISA: 1:20-1:200
Other applications not tested
Optimal dilutions to be determined by the researcher.
Other applications not tested
Optimal dilutions to be determined by the researcher.
Immunogen
Purified toxoid
Grade
Affinity Purified
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Verotoxin II-B subunit antibody
Shiga-like toxins, also called verotoxins, inhibit protein synthesis in eukaryotic cells. These toxins play a role in hemorrhagic colitis and hemolytic uremic syndrome. They damage endothelial cells in both kidney and brain, causing renal failure and neurological complications. The protein structure consists of two domains: the A polypeptide confers the activity, and the B polypeptide pentamer confers the receptor-specific binding. The A polypeptide has an N-glycosidase activity that cleaves an adenine from the 28S rRNA of the 60S cytoplasmic ribosome. This activity renders the 28S rRNA unable to interact with the elongation factors EF-1 and EF-2, thus inhibiting protein synthesis. The B polypeptide forms a pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3). Shiga-like toxins then enter the cells by receptor-mediated endocytosis. The effects of shiga-like toxin 1 and shiga-like toxin 2 have been shown to vary with cell type because Gb3 receptors are present in certain tissues, particularly sensory neurons and renal cells. In those cell types, the receptor concentration and binding is modulated by factors such as tumor necrosis factor (TNF), interleukins, and fatty acid content of the membranes. Shiga-like toxin 1 and shiga-like toxin 2 differ in their effects on various tissues. They have both been shown to induce apoptosis in several different cell types. In addition to inhibiting protein synthesis, shiga- like toxins induce cytokines such as interleukin-1, interleukin-6, and interleukin-8. They have also been shown to induce expression of tumor necrosis factor (TNF), induce F-actin depolymerization, and activate a src kinase. Shiga-like toxin 1 and 2 are from E. coli. Shiga-like toxins are very toxic and should be handled with extreme caution.
Product Categories/Family for anti-Verotoxin II-B subunit antibody
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Product Notes
The Verotoxin II-B subunit (Catalog #AAA601980) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Verotoxin II-B subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Neutralization. Dilution: ELISA: 1:20-1:200 Other applications not tested Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Verotoxin II-B subunit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Verotoxin II-B subunit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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