Rabbit anti-Mouse VEGFR2/KDR Monoclonal Antibody | anti-KDR antibody

Recombinant Anti-VEGFR2/KDR Antibody (PE), Rabbit Monoclonal

Cat. Num. AAA8112231

• Gene Names: Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: VEGFR2/KDR; Monoclonal Antibody; Recombinant Anti-VEGFR2/KDR Antibody (PE); Rabbit Monoclonal; VEGFR2/Flk-1/CD309/KDR Antibody (PE); Rabbit MAb; kinase insert domain receptor; Anti-6130401C07 Antibody; Anti-Flk-1 Antibody; Anti-Flk1 Antibody; Anti-Krd-1 Antibody; Anti-Ly73 Antibody; Anti-sVEGFR-2 Antibody; Anti-VEGFR-2 Antibody; Anti-VEGFR2 Antibody; anti-KDR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 004
• Specificity: Mouse VEGFR2/Flk-1/CD309/KDR
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-KDR antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse VEGFR2/Flk-1/CD309/KDR Protein
• Conjugation: PE
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse VEGFR2/Flk-1/CD309/KDR (rM VEGFR2/Flk-1/CD309/KDR) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Mouse KDR(CD309) expression on bEnd.3 cells. Cells were stained with PE-conjugated anti-Mouse KDR(CD309). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-KDR antibody

VEGFR2 also called KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling are critical to endothelial cell survival, maintenance of the vasculature and alveolar structure, and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.

Product Categories/Family for anti-KDR antibody

Cancer Drug Targets Hemangioblast Markers Receptor Tyrosine Kinases (RTKs)

References

Shibuya M. (2006) Vascular endothelial growth factor (VEGF)-Receptor2: its biological functions, major signaling pathway, and specific ligand VEGF-E. Endothelium. 13(2): 63-9.
Marwick JA, et al. (2010) Cigarette smoke regulates VEGFR2-mediated survival signaling in rat lungs. J Inflamm (Lond). 7(1): 11.
Bruns AF, et al. (2010) Ligand-stimulated VEGFR2 signaling is regulated by co-ordinated trafficking and proteolysis. Traffic. 11(1): 161-74.

NCBI and Uniprot Product Information

• NCBI GI #: 549321
• NCBI GeneID: 16542
• UniProt Accession #: P35918; C5H7S5
• Molecular Weight: 75,230 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain protein receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• NCBI Protein Information: vascular endothelial growth factor receptor 2; kinase NYK; VEGF receptor-2; fetal liver kinase 1; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; vascular endothelial gr
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk-1; Flk1; VEGFR-2; FLK-1
• UniProt Entry Name: VGFR2_MOUSE

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; TK group; VEGFR family

Cellular Component: Golgi apparatus; neuron projection; cell surface; integral to plasma membrane; endoplasmic reticulum; early endosome; integral to membrane; extracellular region; cytosol; lipid raft; cell soma; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; cell junction; nucleus; endosome; external side of plasma membrane

Molecular Function: integrin binding; transferase activity; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of positive chemotaxis; multicellular organismal development; cell maturation; positive regulation of vasodilation; positive regulation of long-term neuronal synaptic plasticity; protein amino acid phosphorylation; calcium ion homeostasis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of mesenchymal cell proliferation; negative regulation of neuron apoptosis; positive regulation of TOR signaling pathway; response to drug; regulation of endothelial cell proliferation; cell fate commitment; embryonic hemopoiesis; regulation of endothelial cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; lymph vessel development; surfactant homeostasis; alveolus development; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of epithelial cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of focal adhesion formation; positive regulation of MAPKKK cascade; ovarian follicle development; positive regulation of cell proliferation; hemopoiesis; angiogenesis; vasculogenesis; cell differentiation; positive regulation of BMP signaling pathway; endothelial cell differentiation; cell migration; male gonad development; response to hypoxia; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; neurite morphogenesis; positive regulation of cell migration; lung development