Mouse anti-Human VCP Monoclonal Antibody | anti-VCP antibody

Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP

Cat. Num. AAA245142

• Gene Names: VCP; p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: VCP; Monoclonal Antibody; Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP; Anti-VCP Antibody (clone 4G9) IHC-plus; 15S Mg(2+)-ATPase p97 subunit; ALS14; TER ATPase; TERA; Valosin containing protein; Yeast Cdc48p homolog; Valosin-containing protein; IBMPFD; p97; Human VCP; anti-VCP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4G9
• Specificity: Recombinant human valosin containing protein
• Purity/Purification: Affinity Purified
• Form/Format: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 806

• Applicable Applications for anti-VCP antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF, IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human VCP.
• Immunogen Type: Recombinant protein
• Immunogen: VCP antibody was raised against recombinant human VCP.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunocytochemistry (ICC)

(Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI)

Western Blot (WB)

(Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9)

Related Product Information for anti-VCP antibody

Valsolin Containing Protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex.

NCBI and Uniprot Product Information

• NCBI GI #: 6005942
• NCBI GeneID: 7415
• NCBI Accession #: NP_009057.1
• NCBI GenBank Nucleotide #: NM_007126.3
• UniProt Accession #: P55072; Q0V924; Q2TAI5; Q969G7; Q9UCD5; B2R5T8
• Molecular Weight: 89,322 Da
• NCBI Official Full Name: transitional endoplasmic reticulum ATPase
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: VCP
• NCBI Official Synonym Symbols: p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• NCBI Protein Information: transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit; epididymis luminal protein 220; epididymis secretory protein Li 70
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: VCP-like ATPase
• UniProt Gene Name: VCP
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: DNA repair, damage; Endoplasmic reticulum; Chaperone; EC 3.6.4.6; Hydrolase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: proteasome complex; nucleoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; polyubiquitin binding; ADP binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; ER to Golgi vesicle-mediated transport; unfolded protein response; protein ubiquitination; DNA repair; regulation of apoptosis; retrograde protein transport, ER to cytosol; establishment of protein localization; bypass DNA synthesis; positive regulation of protein complex assembly; double-strand break repair; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1; Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia