Mouse anti-Human Vasohibin Monoclonal Antibody | anti-VASH1 antibody
Vasohibin
Gene Names
VASH1; KIAA1036
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by Protein G affinity chromatography.
Synonyms
Vasohibin; Monoclonal Antibody; Anti -Vasohibin; anti-VASH1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H175
Specificity
Recognizes human Vasohibin.
Purity/Purification
Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Applicable Applications for anti-VASH1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Direct ELISA and Western Blot.
Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhVasohibin is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rh Vasohibin is ~ 25ng/lane under non-reducing and reducing conditions.
Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhVasohibin is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rh Vasohibin is ~ 25ng/lane under non-reducing and reducing conditions.
Immunogen
NS0-derived rhVasohibin. (aa 2 - 365)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-VASH1 antibody
Vasohibin is an endothelium-derived negative feedback regulator of angiogenesis. The 365 aa, 44kD human Vasohibin shows 93% aa identity with mouse Vasohibin. Vasohibin has no canonical signal sequence, but the full-length form is only associated with cells, while N-terminally cleaved, active 42kD and 36kD forms are both cell-associated and secreted. Minor amounts of C-terminally cleaved 27kD form are inactive and lack the C-terminal heparin-binding basic domain. Vasohibin expression is upregulated by VEGF and FGF2, but this can be modulated by IL-1, TNFalpha or IFNy.
Product Categories/Family for anti-VASH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,957 Da
NCBI Official Full Name
Vasohibin 1
NCBI Official Synonym Full Names
vasohibin 1
NCBI Official Symbol
VASH1
NCBI Official Synonym Symbols
KIAA1036
NCBI Protein Information
vasohibin-1
UniProt Protein Name
Vasohibin-1
Protein Family
UniProt Gene Name
VASH1
UniProt Synonym Gene Names
KIAA1036; VASH
UniProt Entry Name
VASH1_HUMAN
Uniprot Description
VASH1: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Belongs to the vasohibin family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: extracellular space; endoplasmic reticulum; apical part of cell; cytoplasm
Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to wounding; angiogenesis; negative regulation of blood vessel endothelial cell migration; cell cycle arrest
Research Articles on VASH1
Similar Products
Product Notes
The VASH1 vash1 (Catalog #AAA601886) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Vasohibin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vasohibin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Direct ELISA and Western Blot. Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhVasohibin is ~ 1ng/well. Western Blot: 1-2ug/ml detection limit for rh Vasohibin is ~ 25ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the VASH1 vash1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vasohibin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
