Mouse V5 Epitope Monoclonal Antibody
V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5) (MaxLight 550)
Applications
Immunohistochemistry, Immunoprecipitation, immunoassay, Radioimmunoassay, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
V5 Epitope; Monoclonal Antibody; V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag; Paramyxovirus; SV5) (MaxLight 550); PK-TAG; anti-V5 Epitope antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H3115
Specificity
Recognizes recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. (V5-Tag). Detects recombinant proteins including transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.
Applicable Applications for anti-V5 Epitope antibody
FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB)
Immunogen
Paramyxovirus SV5, Simian-Virus 5.
Conjugate
MaxLight550
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Product Categories/Family for anti-V5 Epitope antibody
References
1. Southern, J.A. et al. (1991) Identification of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557. 2. Hanke, T. et al. (1992) Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660. 3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix- antibody antigen (SMAA) complexes as vaccines. Vaccine 11: 1247-1252. 4. Randall, R.E. et al. (1994) Purification of antibody- antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358. 5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J. Virol. Methods 53: 149-156. 6. Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65 Mol. Cell. Biol. 15: 2166-2172. 7. Rodriguez, M.S. et al. (1995) Inducible degradation of IkB in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419. 8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696. 9. Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal EMBO J. 13: 5410-5420. 10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J. Immunol. Methods. 244: 141-150. 11. Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells J. Virol. 75: 3363 - 3370.
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Product Notes
The V5 Epitope (Catalog #AAA6255374) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's V5 Epitope can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB). Researchers should empirically determine the suitability of the V5 Epitope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5 Epitope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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