Mouse anti-Human USP8 Monoclonal Antibody | anti-USP8 antibody

USP8, CT (USP8, KIAA0055, UBPY, Ubiquitin carboxyl-terminal hydrolase 8, Deubiquitinating enzyme 8, Ubiquitin isopeptidase Y, Ubiquitin thioesterase 8, Ubiquitin-specific-processing protease 8)

Cat. Num. AAA643442

• Gene Names: USP8; UBPY; HumORF8
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Ascites; Ascites
• Synonyms: USP8; Monoclonal Antibody; CT (USP8; KIAA0055; UBPY; Ubiquitin carboxyl-terminal hydrolase 8; Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8); Anti -USP8; anti-USP8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 460CT35.1.6
• Specificity: Human
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid, crude ascites with 0.09% sodium azide.

• Applicable Applications for anti-USP8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA.; Dilution: ELISA: 1:1,000; Western Blot: 1:500~4000
• Immunogen: USP8 Mab is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the C-term region of human USP8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(USP8 Antibody (C1072) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the USP8 antibody detected the USP8 protein (arrow).)

Related Product Information for anti-USP8 antibody

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.

Product Categories/Family for anti-USP8 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 545746435
• NCBI GeneID: 9101
• NCBI Accession #: NP_001269978.1
• NCBI GenBank Nucleotide #: NM_001283049.1
• UniProt Accession #: P40818; Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7
• Molecular Weight: 127,523 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 8 isoform b
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: USP8
• NCBI Official Synonym Symbols: UBPY; HumORF8
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8; ubiquitin isopeptidase Y; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: USP8
• UniProt Synonym Gene Names: KIAA0055; UBPY; hUBPy
• UniProt Entry Name: UBP8_HUMAN

NCBI Description

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.

Protein type: Protease; Ubiquitin conjugating system; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: extrinsic to plasma membrane; nucleoplasm; Golgi apparatus; early endosome; cytoplasm; acrosome; midbody; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SH3 domain binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; protein deubiquitination; endosome organization and biogenesis; cytokinesis after mitosis; Ras protein signal transduction