Western Blot (WB)
(Western Blot analysis of HepG2 cells using USP1 Monoclonal Antibody at dilution of 1:2000)
Mouse anti-Human USP1 Monoclonal Antibody | anti-USP1 antibody
USP1 Monoclonal Antibody
Gene Names
USP1; UBP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein A purification
Synonyms
USP1; Monoclonal Antibody; USP1 Monoclonal Antibody; Deubiquitinating enzyme 1; hUBP; Ubiquitin carboxyl terminal hydrolase 1; Ubiquitin carboxyl-terminal hydrolase 1; Ubiquitin specific peptidase 1; Ubiquitin specific protease 1; Ubiquitin thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; UBP1; USP 1; Usp1; anti-USP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
333
Purity/Purification
Protein A purification
Concentration
1mg/mL (varies by lot)
Sequence Length
785
Applicable Applications for anti-USP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-1:10000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Fusion protein of USP1
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of HepG2 cells using USP1 Monoclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using USP1 Monoclonal Antibody at dilution of 1:2000)
Related Product Information for anti-USP1 antibody
USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases. The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment.
Product Categories/Family for anti-USP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 88kDa
Observed: 100kDa
Observed: 100kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 1
NCBI Official Symbol
USP1
NCBI Official Synonym Symbols
UBP
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 1
Protein Family
UniProt Gene Name
USP1
UniProt Synonym Gene Names
hUBP
NCBI Description
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).
Research Articles on USP1
Similar Products
Product Notes
The USP1 usp1 (Catalog #AAA2542110) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The USP1 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-1:10000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the USP1 usp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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