Mouse anti-Human Urokinase Plasminogen Activator Receptor Monoclonal Antibody | anti-PLAUR antibody

Urokinase Plasminogen Activator Receptor (uPAR, CD87)

Cat. Num. AAA605397

• Gene Names: PLAUR; CD87; UPAR; URKR; U-PAR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: Urokinase Plasminogen Activator Receptor; Monoclonal Antibody; Urokinase Plasminogen Activator Receptor (uPAR; CD87); Anti -Urokinase Plasminogen Activator Receptor (uPAR; anti-PLAUR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9B5
• Specificity: Recognizes human uPAR. Reacts with an epitope on domain 1 and differs from that of U2610-04E. Reacts with native uPAR but not with the reduced protein. Inhibits binding to uPAR in solution and on cells. Does not crossreact with murine uPAR, human uPA, human tPA or human PAI-1.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

• Applicable Applications for anti-PLAUR antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1000. Use as a detection antibody.
• Immunogen: Native human Urokinase Plasminogen Activator Receptor (uPAR)
• Culture Medium: RPMI 1640 with 10% fetal calf serum
• Hybridoma: X63-Ag 8.6.5.3 myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PLAUR antibody

Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a glycolipid anchored cell surface protein consisting of three homologous domains. It binds uPA with high affinity and enhances and localizes uPA catalyzed plasminogen activation, and thereby plays a key role in cancer invasion and in several tissue remodelling processes (1-3). In addition uPAR also binds to vitronectin and may thereby have biological functions independent of its role in uPA mediated proteolysis (4,5). Elevated levels of uPAR are associated with poor prognosis in many types of cancer (2,3,6,7).

Product Categories/Family for anti-PLAUR antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 433901
• NCBI GeneID: 5329
• UniProt Accession #: Q03405; Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409; Q6GY32; Q9UPI5
• Molecular Weight: 36,978 Da
• NCBI Official Full Name: urokinase plasminogen activator receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: PLAUR
• NCBI Official Synonym Symbols: CD87; UPAR; URKR; U-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• Protein Family: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR
• UniProt Entry Name: UPAR_HUMAN

NCBI Description

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq]

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane

Molecular Function: protein domain specific binding; protein binding; enzyme binding; U-plasminogen activator receptor activity; receptor activity; receptor binding

Biological Process: fibrinolysis; cellular protein metabolic process; regulation of proteolysis; attachment of GPI anchor to protein; C-terminal protein lipidation; cell motility; signal transduction; post-translational protein modification; chemotaxis; blood coagulation