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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant UCK1 protein (Right) or empty vector (Left) detected with UCK1 antibody)

Mouse UCK1 Monoclonal Antibody | anti-UCK1 antibody

UCK1 antibody

Gene Names
UCK1; URK1
Applications
Western Blot
Purity
UCK1 antibody was purified by affinity chromatography.
Synonyms
UCK1; Monoclonal Antibody; UCK1 antibody; Monoclonal UCK1; Anti-UCK1; Uridine-cytidine kinase 1; URK1; FLJ12255; Cytidine monophosphokinase 1; Uridine monophosphokinase 1; UCK 1; UCK-1; anti-UCK1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6H7
Purity/Purification
UCK1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
277
Applicable Applications for anti-UCK1 antibody
Western Blot (WB)
Application Notes
WB: 1:2000
Biological Significance
Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation ofuridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP).
Cross-Reactivity
Human
Immunogen
UCK1 antibody was raised in mouse using a full length recombinant protein of human UCK1 (NP_113620) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant UCK1 protein (Right) or empty vector (Left) detected with UCK1 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant UCK1 protein (Right) or empty vector (Left) detected with UCK1 antibody)
Related Product Information for anti-UCK1 antibody
Mouse monoclonal UCK1 antibody
Product Categories/Family for anti-UCK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,275 Da
NCBI Official Full Name
uridine-cytidine kinase 1 isoform a
NCBI Official Synonym Full Names
uridine-cytidine kinase 1
NCBI Official Symbol
UCK1
NCBI Official Synonym Symbols
URK1
NCBI Protein Information
uridine-cytidine kinase 1
UniProt Protein Name
Uridine-cytidine kinase 1
Protein Family
UniProt Gene Name
UCK1
UniProt Synonym Gene Names
URK1; UCK 1
UniProt Entry Name
UCK1_HUMAN

NCBI Description

This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012]

Uniprot Description

UCK1: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Nucleotide Metabolism - pyrimidine; EC 2.7.1.48; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytosol

Molecular Function: nucleoside kinase activity; uridine kinase activity; ATP binding

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation

Research Articles on UCK1

Similar Products

Product Notes

The UCK1 uck1 (Catalog #AAA832744) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UCK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000. Researchers should empirically determine the suitability of the UCK1 uck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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