Mouse UCHL3 Monoclonal Antibody | anti-UCHL3 antibody

UCHL3 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3, UCH-L3, Ubiquitin Thioesterase L3)

Cat. Num. AAA633147

• Gene Names: UCHL3; UCH-L3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: UCHL3; Monoclonal Antibody; UCHL3 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3); Anti -UCHL3 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3; anti-UCHL3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10K29
• Specificity: Recognizes huma UCH-L3. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.

• Applicable Applications for anti-UCHL3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Purified, E.coli-derived recombinant human Ubiquitin carboxyterminal hydrolase isozyme 3. (UCH-L3; aa2-230; Accession #P15374).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-UCHL3 antibody

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26-28kD member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230aa in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa40-57 and 178-186. Four potential splice forms have been reported. Two show a four aa deletion between aa15-18, with one also containing a 14aa substitution for aa179-230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa143-230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.

Product Categories/Family for anti-UCHL3 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 5174741
• NCBI GeneID: 7347
• NCBI Accession #: NP_005993.1
• NCBI GenBank Nucleotide #: NM_006002.3
• UniProt Accession #: P15374; Q5TBK8; Q6IBE9; B2R970
• Molecular Weight: 26,183 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3; OTTHUMP00000018509; ubiquitin thiolesterase; ubiquitin thioesterase L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process