Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.)
Mouse Ubiquitin Monoclonal Antibody | anti-UBB antibody
Ubiquitin Antibody: Alkaline Phosphatase
Reactivity
Wide range of species
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Ubiquitin; Monoclonal Antibody; Ubiquitin Antibody: Alkaline Phosphatase; Polyubiquitin B; RPS27A; UBA52; UBB; UBC; ubiquitin B; anti-UBB antibody
Host
Mouse
Reactivity
Wide range of species
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FK2
Form/Format
Protein A Purified
Concentration
10mg/mL (varies by lot)
Sequence Length
229
Applicable Applications for anti-UBB antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
1:1000-10000 (WB)
Conjugate
Alkaline Phosphatase
Storage Buffer
10mM phosphate buffer with 0.15M NaCl, pH7.4, 0.1% sodium azide
Preparation and Storage
-20 degree C
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.)
Related Product Information for anti-UBB antibody
Background Info: Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
Scientific Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Scientific Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Product Categories/Family for anti-UBB antibody
References
1. Chen, J., & Chen, Z. (2013). Regulation of NF-kappaB by ubiquitination. Curr Opin Immunol, (1):4-12. 2. Shaid, S., Brandts, C., Serve, H., & Dikic, I. (2013). Ubiquitination and selective autophagy. Cell Death Differ, 20(1):21-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
25,762 Da
NCBI Official Full Name
polyubiquitin B
NCBI Official Synonym Full Names
ubiquitin B
NCBI Official Symbol
UBB
NCBI Protein Information
polyubiquitin-B; polyubiquitin B
UniProt Protein Name
Polyubiquitin-B
Protein Family
UniProt Gene Name
UBB
UniProt Entry Name
UBB_HUMAN
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Product Notes
The UBB ubb (Catalog #AAA804839) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Antibody: Alkaline Phosphatase reacts with Wide range of species and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). 1:1000-10000 (WB). Researchers should empirically determine the suitability of the UBB ubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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