Mouse anti-Human Tyrosinase Monoclonal Antibody | anti-TYR antibody

Tyrosinase (TYR) (HRP)

Cat. Num. AAA6122487

• Gene Names: TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Tyrosinase; Monoclonal Antibody; Tyrosinase (TYR) (HRP); anti-TYR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: T311
• Specificity: Recognizes human tyrosinase, 70-80kD. Does not cross-react with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas showed tyrosinase in melanotic as well as amelanotic variants.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-TYR antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1-2ug/500ug protein lysate; WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to tyrosinase.
• Conjugate: HRP
• Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human melanoma (10X) stained with Tyrosinase antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human melanoma (20X) stained with Tyrosinase antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.)

Product Categories/Family for anti-TYR antibody

Antibodies; Disease Markers

References

1. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.

NCBI and Uniprot Product Information

• NCBI GI #: 4507753
• NCBI GeneID: 7299
• NCBI Accession #: NP_000363
• NCBI GenBank Nucleotide #: NM_000372
• UniProt Accession #: P14679; Q15675; Q15676; Q15680; Q8TAK4; Q9BYY0; Q9BZX1
• Molecular Weight: 42,914 Da
• NCBI Official Full Name: tyrosinase
• NCBI Official Synonym Full Names: tyrosinase
• NCBI Official Symbol: TYR
• NCBI Official Synonym Symbols: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• NCBI Protein Information: tyrosinase; LB24-AB; SK29-AB; monophenol monooxygenase; oculocutaneous albinism IA; tumor rejection antigen AB
• UniProt Protein Name: Tyrosinase
• Protein Family: Tyrosinase
• UniProt Gene Name: TYR
• UniProt Entry Name: TYRO_HUMAN

Uniprot Description

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.18.1; Cofactor and Vitamin Metabolism - riboflavin; Amino Acid Metabolism - tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; melanosome; integral to membrane; Golgi-associated vesicle

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; monophenol monooxygenase activity

Biological Process: cell proliferation; visual perception; thymus development; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process

Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3