Mouse anti-Human Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40) Monoclonal Antibody | anti-CD40 antibody

Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40)

Cat. Num. AAA2111222

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Tumor Necrosis Factor Receptor Superfamily; Member 5 (CD40); Monoclonal Antibody; Monoclonal Antibody to Tumor Necrosis Factor Receptor Superfamily; TNFRSF5; CDW40; Bp50; P50; B-cell surface antigen CD40; CD40L receptor; anti-CD40 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: C6
• Specificity: The antibody is a mouse monoclonal antibody raised against CD40. It has been selected for its ability to recognize CD40 in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/mL (varies by lot)
• Sequence: Cys26~Gly187
• Sequence Length: 277

• Applicable Applications for anti-CD40 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.2-2ug/mL; 1:500-5000; IHC: 5-20ug/mL; 1:50-200; ICC: 5-20ug/mL; 1:50-200
• Conjugation: None
• Cross Reactivity: Human
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Spleen Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 4507581
• NCBI GeneID: 958
• NCBI Accession #: NP_001241.1
• NCBI GenBank Nucleotide #: NM_001250.5
• UniProt Accession #: P25942
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 5 isoform 1
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5
• UniProt Entry Name: TNR5_HUMAN

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; platelet activation; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; positive regulation of B cell proliferation; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; regulation of immunoglobulin secretion; defense response to virus

Disease: Immunodeficiency With Hyper-igm, Type 3