Mouse Tubulin alpha Monoclonal Antibody | anti-TUBA4A antibody

Tubulin alpha Antibody

Cat. Num. AAA9610329

• Gene Names: TUBA4A; ALS22; TUBA1; H2-ALPHA
• Reactivity: Human, Mouse, Zebrafish, Rabbit
• Applications: Western Blot, ELISA
• Purity: Affinity Purification
• Synonyms: Tubulin alpha; Monoclonal Antibody; Tubulin alpha Antibody; TUBA4A; Alpha-tubulin 1; TUBA1; Testis-specific alpha-tubulin; Tubulin; alpha 4a; H2-ALPHA; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; alpha 1; anti-TUBA4A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Zebrafish, Rabbit
• Clonality: Monoclonal
• Specificity: The alpha-tubulin antibody can detects ebrafish endogenousalpha-tubulin protein.
• Purity/Purification: Affinity Purification
• Form/Format: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 433

• Applicable Applications for anti-TUBA4A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:3000-1:10000
• Immunogen: Peptide from Zebrafish alpha tubulin.
• Subcellular Location: Cytoplasm > Cytoskeleton.

Western Blot (WB)

(Western blot analysis of Tubulin alpha Antibody expression in Hela cells lysates.)

Related Product Information for anti-TUBA4A antibody

Description: Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of alpha-and beta-tubulin.
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit Structure: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CFAP157 (By similarity).
Post-translational Modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Similarity: Belongs to the tubulin family.

NCBI and Uniprot Product Information

• NCBI GI #: 514052659
• NCBI GeneID: 7277
• NCBI Accession #: NP_001265481.1
• NCBI GenBank Nucleotide #: NM_001278552.1
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: Observed: 55 kD; Predicted: 50 kDa
• NCBI Official Full Name: tubulin alpha-4A chain isoform 2
• NCBI Official Synonym Full Names: tubulin alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: ALS22; TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.