Mouse Tubulin, alpha, acetylated Monoclonal Antibody

Tubulin, alpha, acetylated (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Cat. Num. AAA601887

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A chromatography
• Synonyms: Tubulin; alpha; acetylated; Monoclonal Antibody; acetylated (Alpha Tubulin; Tubulin alpha Ubiquitous; H2-Alpha; K-alpha-1; TUBA3; Tubulin alpha 1 Chain; TUBA1; TUBA1A; Tubulin K alpha 1); Anti -Tubulin; anti-Tubulin; acetylated antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 6A205
• Specificity: Recognizes the ~55kD acetylated form of alpha-tubulin.
• Purity/Purification: Affinity Purified; Purified by Protein A chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Tubulin, alpha, acetylated antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot and Immunofluorescence.; Dilution: Western Blot (Human, mouse, rat): 1-3ug/ml; Immunofluorescence (Human, mouse): 1-3ug/ml
• Immunogen: Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Tubulin, alpha, acetylated antibody

The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. Stable microtubules (MTs) are known to contain acetylated alpha-tubulin. Acetylated tubulin is found in the dense networks and bundles of microtubules in the sensory and supporting cells. MTs containing acetylated alpha-tubulin in Ni2+-treated cells were resistant to disassembly induced by nocodazole, and at least partially resistant to cold temperature (0 degrees Celsius), which also depolymerizes MTs. Since acetylated alpha tubulin serves as a marker for the presence of stable MTs, the marked enhancement of alpha tubulin acetylation in Ni2+-treated cells indicates that stabilization of MTs may be an important mechanism by which Ni2+ induces cell injury since stabilized MTs in turn should favor MT bundling. The acetylated microtubules have been shown to be more drug resistant than nonacetylated microtubules. This increased stability is most likely due to the acetylation process.

Product Categories/Family for anti-Tubulin, alpha, acetylated antibody

Antibodies; Abs to Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 393715095
• NCBI GeneID: 7846
• NCBI Accession #: NP_001257329.1
• NCBI GenBank Nucleotide #: NM_001270400.1
• NCBI Official Full Name: tubulin alpha-1A chain isoform 2

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; recycling endosome; cytoplasmic microtubule; cytosol; nucleus

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; organelle organization and biogenesis; mitotic cell cycle; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Lissencephaly 3