Immunohistochemistry (IHC)
(Anti-Tuberin / TSC2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Mouse anti-Mouse, Human Tuberin / TSC2 Monoclonal Antibody | anti-TSC2 antibody
Anti-Tuberin / TSC2 Antibody (clone 249CT19.1.3) IHC-plus
IHC-P (1:50)
WB (1:100)
For long term storage store at -20 degree C.
Immunohistochemistry (IHC)
(Anti-Tuberin / TSC2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Immunohistochemistry (IHC)
(Anti-Tuberin / TSC2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Immunofluorescence (IF)
(Fluorescent image of HeLa cell stained with TSC2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSC2 immunoreactivity is localized to Microtubules significantly.)
Immunofluorescence (IF)
(Fluorescent image of HeLa cell stained with TSC2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSC2 immunoreactivity is localized to Microtubules significantly.)
Western Blot (WB)
(TSC2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The TSC2 antibody detected the TSC2 protein (arrow).)
Western Blot (WB)
(TSC2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The TSC2 antibody detected the TSC2 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.
Protein type: Cell cycle regulation; Tumor suppressor; GAPs; GAPs, misc.; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cytoplasm; cytosol; Golgi apparatus; lysosome; membrane; nucleus; perinuclear region of cytoplasm; TSC1-TSC2 complex
Molecular Function: GTPase activator activity; phosphatase binding; protein binding; protein homodimerization activity; small GTPase binding
Biological Process: cell cycle arrest; endocytosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; glucose import; heart development; innate immune response; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of protein kinase activity; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; neural tube closure; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive chemotaxis; positive regulation of GTPase activity; positive regulation of transcription from RNA polymerase II promoter; protein import into nucleus; protein kinase B signaling cascade; protein localization; regulation of cell cycle; regulation of endocytosis; regulation of insulin receptor signaling pathway; regulation of small GTPase mediated signal transduction; response to hypoxia; transcription initiation from RNA polymerase II promoter; vesicle-mediated transport; viral reproduction
Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2
Research Articles on TSC2
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Product Notes
The TSC2 tsc2 (Catalog #AAA249468) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Tuberin / TSC2 Antibody (clone 249CT19.1.3) IHC-plus reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tuberin / TSC2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB). IF (1:10 - 1:50) IHC-P (1:50) WB (1:100). Researchers should empirically determine the suitability of the TSC2 tsc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tuberin / TSC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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