Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.)
Mouse TrpV3 Monoclonal Antibody | anti-Trpv3 antibody
TrpV3 Antibody: ATTO 488
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation
Synonyms
TrpV3; Monoclonal Antibody; TrpV3 Antibody: ATTO 488; VRL3; vanilloid receptor 2; transient receptor potential cation channel subfamily V member 3; sensitive channel TRPV3; 1110036I10Rik; MGC124324; MGC124325; Trpv3 heat sensitive channel; vanilloid receptor-related osmotically activated channel protein; anti-Trpv3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S15-39
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
791
Applicable Applications for anti-Trpv3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
1ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.)
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filament-like staining in some cells.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filament-like staining in some cells.)
Related Product Information for anti-Trpv3 antibody
Background Info: Detects ~70kDa.
Scientific Background: The TRPV3 protein belongs to a family of non-selective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermo sensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (3, 4).
Scientific Background: The TRPV3 protein belongs to a family of non-selective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermo sensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (3, 4).
Product Categories/Family for anti-Trpv3 antibody
References
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Masamoto Y., Kawabata F., Fushiki T. (2009) Biosci. Biotechnol. Biochem. 73(5): 1021-1027. 4. Xiao R., et al. (2008) J Biol Chem. 283(10): 6162-6174.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90,273 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily V member 3
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily V, member 3
NCBI Official Symbol
Trpv3
NCBI Protein Information
transient receptor potential cation channel subfamily V member 3; heat sensitive channel TRPV3
UniProt Protein Name
Heat sensitive channel TRPV3
Protein Family
UniProt Gene Name
Trpv3
UniProt Entry Name
Q4QYD9_RAT
Research Articles on Trpv3
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Product Notes
The Trpv3 trpv3 (Catalog #AAA803005) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TrpV3 Antibody: ATTO 488 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TrpV3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). 1ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the Trpv3 trpv3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TrpV3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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