Troponin T-Cardiac (95-181 Region) Monoclonal Antibody | anti-Tnnt2 antibody

MAb to Troponin T-Cardiac

Cat. Num. AAA311087

• Gene Names: Tnnt2; Ctt; CTTG; Tnnt3; RATCTTG
• Applications: ELISA, Western Blot
• Purity: >90% pure. Protein A chromatography
• Synonyms: Troponin T-Cardiac (95-181 Region); Monoclonal Antibody; MAb to Troponin T-Cardiac; Monoclonal Antibody to Human Cardiac Troponin T (cTnT); anti-Tnnt2 antibody

• Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1C11
• Specificity: Troponin T-Cardiac (95-181 Region); Human cardiac troponin T. Recognizes an epitope located between amino acid residues 95 and 181 of cTnT. No cross-reactivity with human skeletal Troponin T. Reactive with cTnT from human, mouse, rat and dog.
• Purity/Purification: >90% pure. Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 12.3mg/ml (Sigma protein assay) (varies by lot)

• Applicable Applications for anti-Tnnt2 antibody: EIA/ELISA, Immunoassay Antibody Pairs, Western Blot
• Immunogen: Free Human Cardiac Troponin T
• Affinity Constant: Not determined
• Buffer: PBS, pH 7.4
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Store at 2 to 8 degree C.

Product Categories/Family for anti-Tnnt2 antibody

Monoclonal Antibodies to Cardiac Markers

References

• FEBS, (1993), Volume 315, 1, 25-283

NCBI and Uniprot Product Information

• NCBI GI #: 6981666
• NCBI GeneID: 24837
• NCBI Accession #: NP_036808.1
• NCBI GenBank Nucleotide #: NM_012676.1
• UniProt Accession #: P50753
• Molecular Weight: 35,730 Da
• NCBI Official Full Name: troponin T, cardiac muscle
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: Tnnt2
• NCBI Official Synonym Symbols: Ctt; CTTG; Tnnt3; RATCTTG
• NCBI Protein Information: troponin T, cardiac muscle; cTnT; tnTc; Troponin T cardiac; cardiac troponin T2; troponin T2, cardiac; cardiac muscle troponin T
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: Tnnt2
• UniProt Entry Name: TNNT2_RAT

NCBI Description

tropomyosin-binding subunit of troponin; confers calcium-sensitivy to actinomysin ATPase activity in striated muscle [RGD]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Cellular Component: sarcomere; troponin complex; myofibril; cytoplasm; striated muscle thin filament

Molecular Function: protein binding, bridging; troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: actin crosslink formation; protein heterooligomerization; atrial cardiac muscle morphogenesis; regulation of muscle contraction; metabolic process; heart development; regulation of muscle filament sliding speed; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; response to calcium ion; negative regulation of ATPase activity; cardiac muscle contraction