Mouse Troponin I, cardiac muscle, PHOSPHORYLATED Monoclonal Antibody | anti-TNNI3 antibody

Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

Cat. Num. AAA609006

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• Reactivity: Bovine, Canine, Feline, Goat, Human, Porcine, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Troponin I; cardiac muscle; PHOSPHORYLATED; Monoclonal Antibody; PHOSPHORYLATED (Cardiac Troponin I; TNNI3; TNNC1); Anti -Troponin I; anti-TNNI3 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Feline, Goat, Human, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 10F563
• Specificity: Recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I. Species Crossreactivity: rat, feline, bovine, canine, goat and porcine.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-TNNI3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: For the detection of phosphoproteins, phosphatase inhibitors such as 10mM sodium fluoride and 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Immunogen: Phosphorylated form of human cardiac Troponin I.
• Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TNNI3 antibody

Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts.

Product Categories/Family for anti-TNNI3 antibody

Antibodies; Abs to Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429; Q6FGU5; Q6FGX2
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I type 3 (cardiac)
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a