Mouse anti-Bovine Tropoelastin, Ligamentum Nuchae Monoclonal Antibody
Tropoelastin, Ligamentum Nuchae
Reactivity
Bovine
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Tropoelastin; Ligamentum Nuchae; Monoclonal Antibody; Anti -Tropoelastin; anti-Tropoelastin; Ligamentum Nuchae antibody
Host
Mouse
Reactivity
Bovine
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G3
Specificity
Recognizes bovine tropoelastin and insoluble forms of elastin. Low Species Crossreactivity: human, rabbit, porcine and sheep. This antibody show little if any reactivity towards rat or chicken elastins.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder. Reconstitute with sterile 40-50% glycerol, dH2O, 0.01% Thimerosal.
Applicable Applications for anti-Tropoelastin, Ligamentum Nuchae antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Dilution: ELISA: 1:1000 -1:2000
Western Blot: 1:200 -1:500
Immunoprecipitation: 1:100 -1:200
Immunohistochemistry: 1:50 -1:100
In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Dilution: ELISA: 1:1000 -1:2000
Western Blot: 1:200 -1:500
Immunoprecipitation: 1:100 -1:200
Immunohistochemistry: 1:50 -1:100
In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Immunogen
Bovine ligamentum nuchae tropoelastin.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Tropoelastin, Ligamentum Nuchae antibody
Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored. Elastin is particularly abundant in large elastic blood vessels such as the aorta. It is also very important in the lungs, elastic ligaments, the skin, the bladder, and elastic cartilage.
Tropoelastin is a water-soluble molecule with a molecular weight of approximately 70,000 daltons. Multiple tropoelastin molecules covalently bind together with crosslinks to form the protein elastin that is very prevalent in the body. Tropoelastin is unusual in that it exists in multiple forms, called polymorphs. Tropoelastin is not normally available in its native state, since it becomes crosslinked immediately after its synthesis by the cell and during its export into the extracellular matrix. Tropoelastin confers strength and elasticity to the skin and other organs in the body. A synthetic form of human tropoelastin is made from Genetically Modified organisms for use in wound healing and topical skin care.
Tropoelastin is a water-soluble molecule with a molecular weight of approximately 70,000 daltons. Multiple tropoelastin molecules covalently bind together with crosslinks to form the protein elastin that is very prevalent in the body. Tropoelastin is unusual in that it exists in multiple forms, called polymorphs. Tropoelastin is not normally available in its native state, since it becomes crosslinked immediately after its synthesis by the cell and during its export into the extracellular matrix. Tropoelastin confers strength and elasticity to the skin and other organs in the body. A synthetic form of human tropoelastin is made from Genetically Modified organisms for use in wound healing and topical skin care.
Product Categories/Family for anti-Tropoelastin, Ligamentum Nuchae antibody
Similar Products
Product Notes
The Tropoelastin, Ligamentum Nuchae (Catalog #AAA606788) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tropoelastin, Ligamentum Nuchae reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Tropoelastin, Ligamentum Nuchae can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Dilution: ELISA: 1:1000 -1:2000 Western Blot: 1:200 -1:500 Immunoprecipitation: 1:100 -1:200 Immunohistochemistry: 1:50 -1:100 In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. Researchers should empirically determine the suitability of the Tropoelastin, Ligamentum Nuchae for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tropoelastin, Ligamentum Nuchae, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
