Mouse anti-Human TRF1 / TERF1 Monoclonal Antibody | anti-TRF1 antibody

Mouse Monoclonal [clone BED5] (IgG1) to Human TRF1 / TERF1

Cat. Num. AAA245761

• Gene Names: TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity Purified
• Synonyms: TRF1 / TERF1; Monoclonal Antibody; Mouse Monoclonal [clone BED5] (IgG1) to Human TRF1 / TERF1; Anti-TRF1 / TERF1 Antibody (clone BED5) IHC-plus; TERF1; HTRF1-AS; NIMA-interacting protein 2; PIN2; Telomeric protein Pin2/TRF1; TRBF1; TTAGGG repeat-binding factor 1; T-TRF1; TRF; TRF1; Human TRF1; anti-TRF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BED5
• Specificity: Recognizes TTAGGG repeat binding factor 1 (TRF1) a ubiquitously expressed 60kD protein. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage. Reported to work in Western blotting applications.
• Purity/Purification: Affinity Purified
• Form/Format: PBS, pH 7.4, 0.09% sodium azide. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 439

• Applicable Applications for anti-TRF1 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), ELISA (EIA)
• Application Notes: ELISA, IHC-Fr, IHC-P (10 ug/ml); Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose Histology Positive Control Tissue: Human tonsil.
• Target Species: Human
• Immunogen Description: Recombinant TRF1
• Immunogen Type: Recombinant protein
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TRF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 189409142
• NCBI GeneID: 7013
• NCBI Accession #: NP_059523.2
• NCBI GenBank Nucleotide #: NM_017489.2
• UniProt Accession #: P54274; Q15553; Q8NHT6; Q93029; A7XP29
• Molecular Weight: 48,239 Da
• NCBI Official Full Name: telomeric repeat-binding factor 1 isoform 1
• NCBI Official Synonym Full Names: telomeric repeat binding factor (NIMA-interacting) 1
• NCBI Official Symbol: TERF1
• NCBI Official Synonym Symbols: TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• NCBI Protein Information: telomeric repeat-binding factor 1; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1
• UniProt Protein Name: Telomeric repeat-binding factor 1
• Protein Family: Telomeric DNA-binding factor
• UniProt Gene Name: TERF1
• UniProt Synonym Gene Names: PIN2; TRBF1; TRF; TRF1
• UniProt Entry Name: TERF1_HUMAN

NCBI Description

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; nuclear chromosome, telomeric region; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; microtubule binding; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding; DNA bending activity

Biological Process: response to drug; mitosis; positive regulation of mitosis; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of apoptosis; positive regulation of microtubule polymerization; telomere maintenance via telomerase; positive regulation of mitotic cell cycle; negative regulation of telomerase activity; cell division; negative regulation of DNA replication; telomeric loop formation; mitotic cell cycle spindle assembly checkpoint; age-dependent telomere shortening; G2/M transition of mitotic cell cycle; telomere maintenance; negative regulation of telomere maintenance via telomerase; protein homooligomerization